Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator

Increasing the Sensitivity of Enzyme-Linked Immunosorbent Assay Using Multiplexed Electrokinetic Concentrator

We developed a novel method to increase the sensitivity of standard enzyme-linked immunosorbent assay (ELISA) using a multiplexed electrokinetic concentration chip. The poly(dimethylsiloxane) (PDMS) molecular concentrator was used to trap and collect charged fluorescent product of target-bound enzym...

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Veröffentlicht in:Analytical chemistry (Washington) 2010-04, Vol.82 (8), p.3383-3388
Hauptverfasser: Cheow, Lih Feng, Ko, Sung Hee, Kim, Sung Jae, Kang, Kwan Hyoung, Han, Jongyoon
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Antigens
Biomarkers, Tumor - blood
CA-19-9 Antigen - blood
Chemistry
Dimethylpolysiloxanes - chemistry
Electrochemical methods
Enzyme-Linked Immunosorbent Assay - instrumentation
Enzyme-Linked Immunosorbent Assay - methods
Enzymes
Exact sciences and technology
Fluorescence
Humans
Kinetics
Male
Miscellaneous
Prostate-Specific Antigen - blood
Reaction kinetics
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Zusammenfassung:We developed a novel method to increase the sensitivity of standard enzyme-linked immunosorbent assay (ELISA) using a multiplexed electrokinetic concentration chip. The poly(dimethylsiloxane) (PDMS) molecular concentrator was used to trap and collect charged fluorescent product of target-bound enzyme turnover reaction of ELISA that occurred in a standard 96 well plate. Detection sensitivities of both prostate specific antigen (PSA) and CA 19-9 (a human pancreatic and gastrointestinal cancer marker) ELISAs in serum are enhanced ∼100 fold with a low CV of
ISSN:0003-2700
1520-6882
DOI:10.1021/ac9024335