Ray tracing analysis of the image quality of a high collection efficiency mirror system

Recently, a high collection efficiency mirror system was developed by Watson [Cytometry 10, 681-688 (1989)] to increase the sensitivity of low level fluorescence detection. The mirror system consists of an ellipsoidal imaging mirror and spherical backreflecting mirror. The fluorescing sample is loca...

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Veröffentlicht in:	Applied Optics 1990-10, Vol.29 (28), p.4255-4258
Hauptverfasser:	SEITZINGER, N. K, MARTIN, J. C, KELLER, R. A
Format:	Artikel
Sprache:	eng
Schlagworte:	440600 - Optical Instrumentation- (1990-) COUNTING TECHNIQUES EFFICIENCY EMISSION SPECTROSCOPY Exact sciences and technology FLUORESCENCE SPECTROSCOPY Fundamental areas of phenomenology (including applications) Lenses, prisms, and mirrors LOW LEVEL COUNTING MIRRORS Optical elements, devices, and systems OPTICAL SYSTEMS Optics OTHER INSTRUMENTATION Physics SPECTROSCOPY
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Zusammenfassung:	Recently, a high collection efficiency mirror system was developed by Watson [Cytometry 10, 681-688 (1989)] to increase the sensitivity of low level fluorescence detection. The mirror system consists of an ellipsoidal imaging mirror and spherical backreflecting mirror. The fluorescing sample is located at one focus of the ellipsoid, and its image is formed at the other focus. In this paper we evaluate the image quality of this geometry using a PC-based ray tracing program. The analysis demonstrates high collection efficiency but poor image quality. The effect of poor image quality on single molecule detection is discussed.
ISSN:	0003-6935 1559-128X 1539-4522
DOI:	10.1364/AO.29.004255