Mucilaginibacter rigui sp. nov., isolated from wetland freshwater, and emended description of the genus Mucilaginibacter

1 Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 540-742, Republic of Korea 2 Department of Dental Hygiene, Gwangju Health College University, Gwangju, 506-701, Republic of Korea Correspondence Chi Nam Seong scnu{at}scnu.ac.kr A non-motile, rod-shaped bacterium, designated strain WPCB133 T , was isolated from freshwater collected from the Woopo wetland (Republic of Korea). Cells were Gram-reaction-negative, aerobic and catalase- and oxidase-positive. The major fatty acids were iso-C 15 : 0 and anteiso-C 15 : 0 . The strain contained MK-7 as the major isoprenoid quinone. The DNA G+C content was 47 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain WPCB133 T forms an independent lineage within the genus Mucilaginibacter . Strain WPCB133 T was distantly related to Mucilaginibacter kameinonensis SCK T (94.7 % sequence similarity), Mucilaginibacter paludis TPT56 T (94.5 %) and Mucilaginibacter gracilis TPT18 T (94.4 %). Phenotypic characteristics distinguished strain WPCB133 T from members of the genus Mucilaginibacter . On the basis of evidence presented in this study, strain WPCB133 T represents a novel species of the genus Mucilaginibacter , for which the name Mucilaginibacter rigui sp. nov. is proposed. The type strain is WPCB133 T (=KCTC 12534 T =NBRC 101115 T ). An emended description of the genus Mucilaginibacter is also proposed. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain WPCB133 T is EU747841 . Scanning and transmission electron micrographs of cells of strain WPCB133 T are available with the online version of this paper.