Pandoraea thiooxydans sp. nov., a facultatively chemolithotrophic, thiosulfate-oxidizing bacterium isolated from rhizosphere soils of sesame (Sesamum indicum L.)

1 Organic Agriculture Division, National Academy of Agricultural Science (formerly National Institute of Agricultural Science and Technology), Rural Development Administration, Suwon 441-707, Republic of Korea 2 Department of Agricultural Chemistry, Chungbuk National University, Cheongju 361-763, Republic of Korea 3 Department of Agricultural Biology, Chungbuk National University, Cheongju 361-763, Republic of Korea 4 Korean Agricultural Culture Collection, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea 5 Department of Life Science, Chung-Ang University, Seoul 156-756, Republic of Korea Correspondence Tong Min Sa tomsa{at}chungbuk.ac.kr A facultatively chemolithoautotrophic, thiosulfate-oxidizing, Gram-negative, aerobic, motile, rod-shaped bacterial strain, designated ATSB16 T , was isolated from rhizosphere soils of sesame ( Sesamum indicum L.). 16S rRNA gene sequence analysis demonstrated that this strain was closely related to Pandoraea pnomenusa LMG 18087 T (96.7 % similarity), P. pulmonicola LMG 18016 T (96.5 %), P. apista LMG 16407 T (96.2 %), P. norimbergensis LMG 18379 T (96.1 %) and P. sputorum LMG 18819 T (96.0 %). Strain ATSB16 T shared 96.0–96.4 % sequence similarity with four unnamed genomospecies of Pandoraea . The major cellular fatty acids of the strain ATSB16 T were C 17 : 0 cyclo (33.0 %) and C 16 : 0 (30.6 %). Q-8 was the predominant respiratory quinone. The major polar lipids were phosphatidylmethylethanolamine, diphosphatidylglycerol, phosphatidylethanolamine and two unidentified aminophospholipids. Hydroxyputrescine and putrescine were the predominant polyamines. The genomic DNA G+C content of the strain was 64.0 mol%. On the basis of the results obtained from this study, strain ATSB16 T represents a novel species of the genus Pandoraea , for which the name Pandoraea thiooxydans sp. nov. is proposed. The type strain is ATSB16 T (=KACC 12757 T =LMG 24779 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain ATSB16 T is EF397578 .