Loop-Mediated Isothermal Amplification Integrated on Microfluidic Chips for Point-of-Care Quantitative Detection of Pathogens

This work shows that loop-mediated isothermal amplification (LAMP) of nucleic acid can be integrated in an eight-channel microfluidic chip for readout either by the naked eye (as a result of the insoluble byproduct pyrophosphate generating during LAMP amplification) or via absorbance measured by an...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-04, Vol.82 (7), p.3002-3006
Hauptverfasser:	Fang, Xueen, Liu, Yingyi, Kong, Jilie, Jiang, Xingyu
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemistry Deoxyribonucleic acid Diphosphates - chemistry DNA DNA, Viral - analysis Exact sciences and technology Fiber optics General, instrumentation Herpesvirus 1, Suid - genetics Integration Microfluidic Analytical Techniques - methods Nucleic Acid Amplification Techniques - methods Point-of-Care Systems Real time Spectrometric and optical methods
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