Peritoneal macrophages from patients on continuous ambulatory peritoneal dialysis show a differential secretion of prostanoids and interleukin-1β

In vitro secretion of the prostanoids PGE 2 and PGI 2 and of the cytokine IL-1β by peritoneal macrophages obtained from CAPD patients during episodes of peritonitis and infection free periods, was determined, after culturing with or without 5 μg/ml of LPS. The release of PGE 2 and PGI 2 as measured by its stable metabolite 6-keto-PGFα was determined in 10 episodes of peritonitis and 10 infection free periods. IL-1β release was determined in 14 episodes of peritonitis and 20 infection free periods. PGI 2 release from macrophages declined sharply during peritonitis both in the absence and presence of LPS in the culture medium (p