An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles

An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles

An operationally simple colorimetric way for measuring hyaluronidase activity was developed using cysteamine-bound gold nanoparticles. The addition of gold nanoparticles into hyaluronidase-containing solutions resulted in color changes, which could easily be observed with the naked eye or a UV/Vis s...

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Veröffentlicht in:Analyst (London) 2009-01, Vol.134 (7), p.1291-1293
Hauptverfasser: KIM, Jung-Won, JOONG HYUN KIM, SANG JEON CHUNG, BONG HYUN CHUNG
Format: Artikel
Sprache:eng
Schlagworte:
Absorption
Analytical chemistry
Chemistry
Colorimetry - methods
Exact sciences and technology
Gold - chemistry
Hyaluronoglucosaminidase - analysis
Hyaluronoglucosaminidase - metabolism
Metal Nanoparticles - chemistry
Spectrometric and optical methods
Time Factors
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Zusammenfassung:An operationally simple colorimetric way for measuring hyaluronidase activity was developed using cysteamine-bound gold nanoparticles. The addition of gold nanoparticles into hyaluronidase-containing solutions resulted in color changes, which could easily be observed with the naked eye or a UV/Vis spectrophotometer.
ISSN:0003-2654
1364-5528
DOI:10.1039/b822917a