Small-Molecule Affinity Ligands for Protein Purification: Combined Computational Enrichment and Automated In-line Screening of an Optically Encoded Library

Screening with codes: A versatile methodology for the development of small‐molecule affinity chromatography ligands for protein targets is presented. The combination of computational enrichment with automated screening and affinity mapping of an optically encoded combinatorial library allows identification of a novel set of ligands for the single‐step purification of human growth hormone (see picture).