The use of capillary electrophoresis in a micropreparative mode: Methods and applications

The ability to collect sufficient quantities of analytes from capillary electrophoresis for subsequent analyses is demonstrated. Fractions collected have been analyzed using the following techniques: capillary electrophoresis, mass spectrometry, and protein sequencing. Fractions can be collected dir...

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Veröffentlicht in:	Analytical biochemistry 1992-11, Vol.206 (2), p.382-388
Hauptverfasser:	Albin, Michael, Chen, Shiaw-Min, Louie, Andrea, Pairaud, Claire, Colburn, Joel, Wiktorowicz, John
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Analytical biochemistry: general aspects, technics, instrumentation Analytical, structural and metabolic biochemistry Biological and medical sciences Capillary Action capillary electrophoresis Chromatography, High Pressure Liquid - methods Electrophoresis - instrumentation Electrophoresis - methods Endopeptidases Fundamental and applied biological sciences. Psychology isolation methodology Molecular Sequence Data Peptide Fragments - chemistry Peptide Fragments - isolation & purification Peptides - chemistry Peptides - isolation & purification preparative proteins Proteins - chemistry Proteins - isolation & purification
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Beschreibung
Zusammenfassung:	The ability to collect sufficient quantities of analytes from capillary electrophoresis for subsequent analyses is demonstrated. Fractions collected have been analyzed using the following techniques: capillary electrophoresis, mass spectrometry, and protein sequencing. Fractions can be collected directly into small volumes of buffer or directly onto membrane surfaces. Relevant parameters such as capillary diameter, mass loading, and separation parameters are addressed.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(92)90382-H