E-proteins directly regulate expression of activation-induced deaminase in mature B cells
Activated mature B cells in which the DNA-binding activity of E-proteins has been disrupted fail to undergo class switch recombination. Here we show that activated B cells overexpressing the antagonist helix-loop-helix protein Id3 do not induce expression of the murine Aicda gene encoding activation...
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Veröffentlicht in: | Nature immunology 2003-06, Vol.4 (6), p.586-593 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Activated mature B cells in which the DNA-binding activity of E-proteins has been disrupted fail to undergo class switch recombination. Here we show that activated B cells overexpressing the antagonist helix-loop-helix protein Id3 do not induce expression of the murine
Aicda
gene encoding activation-induced deaminase (AID). A highly conserved intronic regulatory element in
Aicda
binds E-proteins both
in vitro
and
in vivo
. The transcriptional activity of this element is regulated by E-proteins. We show that the enforced expression of AID in cells overexpressing Id3 partially restores class switch recombination. Taken together, our observations link helix-loop-helix activity and
Aicda
gene expression in a common pathway, in which E-protein activity is required for the efficient induction of
Aicda
transcription. |
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ISSN: | 1529-2908 1529-2916 |
DOI: | 10.1038/ni923 |