Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis
The role of indirect binding of host proteins through glycosaminoglycans (GAGs) on adherence and internalization of Streptococcus uberis to bovine mammary epithelial cells was evaluated. Preincubation of S. uberis with GAGs followed by incubation with fetal bovine serum (FBS), bovine milk or milk pr...
Gespeichert in:
| Veröffentlicht in: | Veterinary microbiology 2003-07, Vol.94 (2), p.131-141 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 141 |
|---|---|
| container_issue | 2 |
| container_start_page | 131 |
| container_title | Veterinary microbiology |
| container_volume | 94 |
| creator | Almeida, Raul A. Luther, Douglas A. Nair, Rajeev Oliver, Stephen P. |
| description | The role of indirect binding of host proteins through glycosaminoglycans (GAGs) on adherence and internalization of
Streptococcus uberis to bovine mammary epithelial cells was evaluated. Preincubation of
S. uberis with GAGs followed by incubation with fetal bovine serum (FBS), bovine milk or milk proteins resulted in greater adherence to and internalization of
S. uberis into mammary epithelial cells than observed in untreated controls. Highest values were detected, when final incubation was done with milk. Greater adherence to and internalization into mammary epithelial cells were observed when heparin sulfate (HEP) and milk were used compared with any other GAG and FBS. When individual milk proteins were used, greatest adherence and internalization were observed when
S. uberis strains were pretreated with HEP followed by treatment with β-casein. The findings of this study illustrate a pathogenic strategy of
S. uberis that may occur during the very early stages of infection. |
| doi_str_mv | 10.1016/S0378-1135(03)00078-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73325193</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0378113503000786</els_id><sourcerecordid>73325193</sourcerecordid><originalsourceid>FETCH-LOGICAL-c422t-ab235a6048f4fbe4baac7ac20682926b466acc4035c1c09b946001a7e7665faa3</originalsourceid><addsrcrecordid>eNqFkUtv1TAQhS0EoreFnwDyBlQWAb_iJGwQVLykSiwKa8txJvcaEjt4HET_Pb4P0WU3tsf65szoHEKecfaaM67f3DDZtBXnsr5k8hVjrFT6AdnwtpGVqJV4SDb_kTNyjvizQKrT7DE546JpuWr5hvz94MPgw5bGke4iZrqdbl1EO_sQ908bkNow0NlPv-iSYgYf8C1dIqLvJ6AplsMHmndAF5t3cQsB0ONe7yYnWHJ00bkV6dpDKv-zxeyzxyfk0WgnhKen-4L8-PTx-9WX6vrb569X768rp4TIle2FrK1mqh3V2IPqrXWNdYLpVnRC90pr65xisnbcsa7vlGaM2wYarevRWnlBXh51y_K_V8BsZo8OpskGiCuaRkpR807eCxZj61bproD1EXSpuJBgNEvys023hjOzz8YcsjF74w2T5pCN0aXv-WnA2s8w3HWdwijAixNg0dlpTDY4j3ecaqWsD9y7IwfFtz8ekkHnITgYfAKXzRD9Pav8Az48rV8</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>18758469</pqid></control><display><type>article</type><title>Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Almeida, Raul A. ; Luther, Douglas A. ; Nair, Rajeev ; Oliver, Stephen P.</creator><creatorcontrib>Almeida, Raul A. ; Luther, Douglas A. ; Nair, Rajeev ; Oliver, Stephen P.</creatorcontrib><description>The role of indirect binding of host proteins through glycosaminoglycans (GAGs) on adherence and internalization of
Streptococcus uberis to bovine mammary epithelial cells was evaluated. Preincubation of
S. uberis with GAGs followed by incubation with fetal bovine serum (FBS), bovine milk or milk proteins resulted in greater adherence to and internalization of
S. uberis into mammary epithelial cells than observed in untreated controls. Highest values were detected, when final incubation was done with milk. Greater adherence to and internalization into mammary epithelial cells were observed when heparin sulfate (HEP) and milk were used compared with any other GAG and FBS. When individual milk proteins were used, greatest adherence and internalization were observed when
S. uberis strains were pretreated with HEP followed by treatment with β-casein. The findings of this study illustrate a pathogenic strategy of
S. uberis that may occur during the very early stages of infection.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/S0378-1135(03)00078-6</identifier><identifier>PMID: 12781481</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Bacterial adherence ; Bacterial Adhesion - physiology ; Bacteriology ; Biological and medical sciences ; Cattle ; Cattle-bacteria ; Enzyme-Linked Immunosorbent Assay - veterinary ; Female ; Fundamental and applied biological sciences. Psychology ; Glycosaminoglycans ; Glycosaminoglycans - metabolism ; Heparin - analogs & derivatives ; Heparin - metabolism ; Mammary Glands, Animal - microbiology ; Mastitis ; Mastitis, Bovine - etiology ; Mastitis, Bovine - microbiology ; Microbiology ; Milk - microbiology ; Milk - physiology ; Milk Proteins - metabolism ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Proteoglycans - metabolism ; Streptococcal Infections - etiology ; Streptococcal Infections - microbiology ; Streptococcal Infections - veterinary ; Streptococcus - pathogenicity ; Streptococcus - physiology ; Streptococcus uberis</subject><ispartof>Veterinary microbiology, 2003-07, Vol.94 (2), p.131-141</ispartof><rights>2003 Elsevier Science B.V.</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-ab235a6048f4fbe4baac7ac20682926b466acc4035c1c09b946001a7e7665faa3</citedby><cites>FETCH-LOGICAL-c422t-ab235a6048f4fbe4baac7ac20682926b466acc4035c1c09b946001a7e7665faa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0378113503000786$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14833581$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12781481$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Almeida, Raul A.</creatorcontrib><creatorcontrib>Luther, Douglas A.</creatorcontrib><creatorcontrib>Nair, Rajeev</creatorcontrib><creatorcontrib>Oliver, Stephen P.</creatorcontrib><title>Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>The role of indirect binding of host proteins through glycosaminoglycans (GAGs) on adherence and internalization of
Streptococcus uberis to bovine mammary epithelial cells was evaluated. Preincubation of
S. uberis with GAGs followed by incubation with fetal bovine serum (FBS), bovine milk or milk proteins resulted in greater adherence to and internalization of
S. uberis into mammary epithelial cells than observed in untreated controls. Highest values were detected, when final incubation was done with milk. Greater adherence to and internalization into mammary epithelial cells were observed when heparin sulfate (HEP) and milk were used compared with any other GAG and FBS. When individual milk proteins were used, greatest adherence and internalization were observed when
S. uberis strains were pretreated with HEP followed by treatment with β-casein. The findings of this study illustrate a pathogenic strategy of
S. uberis that may occur during the very early stages of infection.</description><subject>Animals</subject><subject>Bacterial adherence</subject><subject>Bacterial Adhesion - physiology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cattle-bacteria</subject><subject>Enzyme-Linked Immunosorbent Assay - veterinary</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycosaminoglycans</subject><subject>Glycosaminoglycans - metabolism</subject><subject>Heparin - analogs & derivatives</subject><subject>Heparin - metabolism</subject><subject>Mammary Glands, Animal - microbiology</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - etiology</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Microbiology</subject><subject>Milk - microbiology</subject><subject>Milk - physiology</subject><subject>Milk Proteins - metabolism</subject><subject>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</subject><subject>Proteoglycans - metabolism</subject><subject>Streptococcal Infections - etiology</subject><subject>Streptococcal Infections - microbiology</subject><subject>Streptococcal Infections - veterinary</subject><subject>Streptococcus - pathogenicity</subject><subject>Streptococcus - physiology</subject><subject>Streptococcus uberis</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1TAQhS0EoreFnwDyBlQWAb_iJGwQVLykSiwKa8txJvcaEjt4HET_Pb4P0WU3tsf65szoHEKecfaaM67f3DDZtBXnsr5k8hVjrFT6AdnwtpGVqJV4SDb_kTNyjvizQKrT7DE546JpuWr5hvz94MPgw5bGke4iZrqdbl1EO_sQ908bkNow0NlPv-iSYgYf8C1dIqLvJ6AplsMHmndAF5t3cQsB0ONe7yYnWHJ00bkV6dpDKv-zxeyzxyfk0WgnhKen-4L8-PTx-9WX6vrb569X768rp4TIle2FrK1mqh3V2IPqrXWNdYLpVnRC90pr65xisnbcsa7vlGaM2wYarevRWnlBXh51y_K_V8BsZo8OpskGiCuaRkpR807eCxZj61bproD1EXSpuJBgNEvys023hjOzz8YcsjF74w2T5pCN0aXv-WnA2s8w3HWdwijAixNg0dlpTDY4j3ecaqWsD9y7IwfFtz8ekkHnITgYfAKXzRD9Pav8Az48rV8</recordid><startdate>20030701</startdate><enddate>20030701</enddate><creator>Almeida, Raul A.</creator><creator>Luther, Douglas A.</creator><creator>Nair, Rajeev</creator><creator>Oliver, Stephen P.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20030701</creationdate><title>Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis</title><author>Almeida, Raul A. ; Luther, Douglas A. ; Nair, Rajeev ; Oliver, Stephen P.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-ab235a6048f4fbe4baac7ac20682926b466acc4035c1c09b946001a7e7665faa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Bacterial adherence</topic><topic>Bacterial Adhesion - physiology</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cattle-bacteria</topic><topic>Enzyme-Linked Immunosorbent Assay - veterinary</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycosaminoglycans</topic><topic>Glycosaminoglycans - metabolism</topic><topic>Heparin - analogs & derivatives</topic><topic>Heparin - metabolism</topic><topic>Mammary Glands, Animal - microbiology</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - etiology</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Microbiology</topic><topic>Milk - microbiology</topic><topic>Milk - physiology</topic><topic>Milk Proteins - metabolism</topic><topic>Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains</topic><topic>Proteoglycans - metabolism</topic><topic>Streptococcal Infections - etiology</topic><topic>Streptococcal Infections - microbiology</topic><topic>Streptococcal Infections - veterinary</topic><topic>Streptococcus - pathogenicity</topic><topic>Streptococcus - physiology</topic><topic>Streptococcus uberis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Almeida, Raul A.</creatorcontrib><creatorcontrib>Luther, Douglas A.</creatorcontrib><creatorcontrib>Nair, Rajeev</creatorcontrib><creatorcontrib>Oliver, Stephen P.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Almeida, Raul A.</au><au>Luther, Douglas A.</au><au>Nair, Rajeev</au><au>Oliver, Stephen P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2003-07-01</date><risdate>2003</risdate><volume>94</volume><issue>2</issue><spage>131</spage><epage>141</epage><pages>131-141</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>The role of indirect binding of host proteins through glycosaminoglycans (GAGs) on adherence and internalization of
Streptococcus uberis to bovine mammary epithelial cells was evaluated. Preincubation of
S. uberis with GAGs followed by incubation with fetal bovine serum (FBS), bovine milk or milk proteins resulted in greater adherence to and internalization of
S. uberis into mammary epithelial cells than observed in untreated controls. Highest values were detected, when final incubation was done with milk. Greater adherence to and internalization into mammary epithelial cells were observed when heparin sulfate (HEP) and milk were used compared with any other GAG and FBS. When individual milk proteins were used, greatest adherence and internalization were observed when
S. uberis strains were pretreated with HEP followed by treatment with β-casein. The findings of this study illustrate a pathogenic strategy of
S. uberis that may occur during the very early stages of infection.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>12781481</pmid><doi>10.1016/S0378-1135(03)00078-6</doi><tpages>11</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1135 |
| ispartof | Veterinary microbiology, 2003-07, Vol.94 (2), p.131-141 |
| issn | 0378-1135 1873-2542 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73325193 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Bacterial adherence Bacterial Adhesion - physiology Bacteriology Biological and medical sciences Cattle Cattle-bacteria Enzyme-Linked Immunosorbent Assay - veterinary Female Fundamental and applied biological sciences. Psychology Glycosaminoglycans Glycosaminoglycans - metabolism Heparin - analogs & derivatives Heparin - metabolism Mammary Glands, Animal - microbiology Mastitis Mastitis, Bovine - etiology Mastitis, Bovine - microbiology Microbiology Milk - microbiology Milk - physiology Milk Proteins - metabolism Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Proteoglycans - metabolism Streptococcal Infections - etiology Streptococcal Infections - microbiology Streptococcal Infections - veterinary Streptococcus - pathogenicity Streptococcus - physiology Streptococcus uberis |
| title | Binding of host glycosaminoglycans and milk proteins: possible role in the pathogenesis of Streptococcus uberis mastitis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-30T06%3A18%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Binding%20of%20host%20glycosaminoglycans%20and%20milk%20proteins:%20possible%20role%20in%20the%20pathogenesis%20of%20Streptococcus%20uberis%20mastitis&rft.jtitle=Veterinary%20microbiology&rft.au=Almeida,%20Raul%20A.&rft.date=2003-07-01&rft.volume=94&rft.issue=2&rft.spage=131&rft.epage=141&rft.pages=131-141&rft.issn=0378-1135&rft.eissn=1873-2542&rft.coden=VMICDQ&rft_id=info:doi/10.1016/S0378-1135(03)00078-6&rft_dat=%3Cproquest_cross%3E73325193%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=18758469&rft_id=info:pmid/12781481&rft_els_id=S0378113503000786&rfr_iscdi=true |