Overexpression of Cold Shock Protein A of Psychromonas arctica KOPRI 22215 Confers Cold-Resistance
A polar bacterium was isolated from Arctic sea sediments and identified as Psychromonas artica, based on 16S rDNA sequence. Psychromonas artica KOPRI 22215 has an optimal growth temperature of 10 °C and a maximum growth temperature of 25 °C, suggesting this bacterium is a psychrophile. Cold shock pr...
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Veröffentlicht in: | The Protein Journal 2010-02, Vol.29 (2), p.136-142 |
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Sprache: | eng |
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Zusammenfassung: | A polar bacterium was isolated from Arctic sea sediments and identified as
Psychromonas artica,
based on 16S rDNA sequence.
Psychromonas artica
KOPRI 22215 has an optimal growth temperature of 10 °C and a maximum growth temperature of 25 °C, suggesting this bacterium is a psychrophile. Cold shock proteins (Csps) are induced upon temperature downshift by more than 10 °C. Functional studies have researched mostly Csps of a mesophilic bacterium
Escherichia coli
, but not on those of psychrophilic bacteria. In an effort to understand the molecular mechanisms of psychrophilic bacteria that allow it withstand freezing environments, we cloned a gene encoding a cold shock protein from
P. artica
KOPRI 22215 (CspA
Pa
) using the conserved sequences in
csp
genes. The 204 bp-long ORF encoded a protein of 68 amino acids, sharing 56% homology to previously reported
E. coli
CspA protein. When CspA
Pa
was overexpressed in
E. coli
, it caused cell growth-retardation and morphological elongation. Interestingly, overexpression of CspA
Pa
drastically increased the host’s cold-resistance by more than ten times, suggesting the protein aids survival in polar environments. |
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ISSN: | 1572-3887 1573-4943 1875-8355 |
DOI: | 10.1007/s10930-010-9233-9 |