Temperature Effects on the Rate of Ty Transposition

Temperature Effects on the Rate of Ty Transposition

An assay has been developed to measure the rate of transposition of the transposable element Ty in Saccharomyces cerevisiae. The assay is based on the altered expression of the glucose-repressible alcohol dehydrogenase gene of yeast upon insertion of a Ty in front of this gene. By this assay the tra...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Science (American Association for the Advancement of Science) 1984-10, Vol.226 (4670), p.53-55
Hauptverfasser: Paquin, Charlotte E., Williamson, Valerie M.
Format: Artikel
Sprache:eng
Schlagworte:
Cell culture techniques
Cultured cells
Enzymes
Genetic loci
Genetic mutation
Genetic transposition
Low temperature
Molecular genetics
Plant cells
Plant cells and tissues
Plant genetics
Retrotransposons
Transposons
Yeasts
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:An assay has been developed to measure the rate of transposition of the transposable element Ty in Saccharomyces cerevisiae. The assay is based on the altered expression of the glucose-repressible alcohol dehydrogenase gene of yeast upon insertion of a Ty in front of this gene. By this assay the transposition rate of Ty elements was found to increase approximately 100-fold at temperatures lower than 30° C, the optimum growth temperature for Saccharomyces cerevisiae.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.226.4670.53