Evidence for Mixed Cytoplasm in Heterocaryons of Colletotrichum lagenarium

Spores of race 2 but not of race 1 of Colletotrichum lagenarium are ingested by myxamoebas of strain NE-30 of the slime mold Acrasis rosea. Sporulating colonies of three heterocaryons of C. lagenarium involving auxotrophic strains with different color markers of races 1 and 2 were inoculated with myxamoebas. Although spores of race 1 produced on homocaryons were not ingested by the myxamoebas, they were ingested when produced on heterocaryons involving race 2. Spores from sectors of the heterocaryons yielded colonies of race 1; spores from these colonies were not ingested by the myxamoebas.