Development of a cps-based multiplex PCR for typing of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5

A cps-based multiplex PCR for typing of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5 was developed. This method should be specific and practical in Japan where more than 88% of isolates are serotypes 1, 2 or 5.

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of Veterinary Medical Science 2010, Vol.72(5), pp.653-655
1. Verfasser:	Ito, H., National Inst. of Animal Health, Tsukuba, Ibaraki (Japan)
Format:	Artikel
Sprache:	eng
Schlagworte:	ACTINOBACILLUS PLEUROPNEUMONIAE Actinobacillus pleuropneumoniae - classification Actinobacillus pleuropneumoniae - genetics Bacterial Typing Techniques - methods CERDO cps DNA Primers DNA, Bacterial - genetics http://www.fao.org/aos/agrovoc#c_27110 http://www.fao.org/aos/agrovoc#c_32699 http://www.fao.org/aos/agrovoc#c_34079 http://www.fao.org/aos/agrovoc#c_4788 http://www.fao.org/aos/agrovoc#c_6097 http://www.fao.org/aos/agrovoc#c_7555 METHODE METHODS METODOS multiplex PCR PCR POLISACARIDOS POLYHOLOSIDE Polymerase Chain Reaction - methods POLYSACCHARIDES PORCIN SEROTIPOS SEROTYPE SEROTYPES serotypes 1, 2 and 5 SWINE typing
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Beschreibung
Zusammenfassung:	A cps-based multiplex PCR for typing of Actinobacillus pleuropneumoniae serotypes 1, 2 and 5 was developed. This method should be specific and practical in Japan where more than 88% of isolates are serotypes 1, 2 or 5.
ISSN:	0916-7250 1347-7439
DOI:	10.1292/jvms.09-0529