Dynamic Glycosylation of the Transcription Factor CREB: A Potential Role in Gene Regulation

We report that CREB (cyclic AMP-responsive element-binding protein), a transcription factor essential for long-term memory, is O-GlcNAc glycosylated in the mammalian brain. Glycosylation occurs at two sites within the Q2 domain and disrupts the interaction between CREB and TAFII130, thereby repressi...

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Veröffentlicht in:	Journal of the American Chemical Society 2003-06, Vol.125 (22), p.6612-6613
Hauptverfasser:	Lamarre-Vincent, Nathan, Hsieh-Wilson, Linda C
Format:	Artikel
Sprache:	eng
Schlagworte:	Acetylglucosamine - chemistry Acetylglucosamine - metabolism Analytical chemistry Animals Brain - metabolism Brain - physiology Chemistry Cyclic AMP Response Element-Binding Protein - metabolism Cyclic AMP Response Element-Binding Protein - physiology Exact sciences and technology Gene Expression Regulation - physiology Glycosylation HeLa Cells Humans Miscellaneous N-Acetyllactosamine Synthase - chemistry N-Acetyllactosamine Synthase - metabolism Rats Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization TATA-Binding Protein Associated Factors - metabolism TATA-Binding Protein Associated Factors - physiology Transcription Factor TFIID - metabolism Transcription Factor TFIID - physiology Transcriptional Activation - physiology
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Zusammenfassung:	We report that CREB (cyclic AMP-responsive element-binding protein), a transcription factor essential for long-term memory, is O-GlcNAc glycosylated in the mammalian brain. Glycosylation occurs at two sites within the Q2 domain and disrupts the interaction between CREB and TAFII130, thereby repressing the transcriptional activity of CREB in vitro. These findings have important implications for the role of O-GlcNAc glycosylation in gene regulation, and they provide a link between O-GlcNAc and information storage processes in the brain.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja028200t