Measurement of the activity of individual respiratory chain complexes in isolated fibroblast mitochondria
We have developed methods for preparing a mitochondrial fraction from six to eight 80-cm super(2) flasks and optimizing the measurement of the activity of individual respiratory chain complexes. We have demonstrated that these techniques are sufficiently sensitive to detect respiratory chain defects...
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| Veröffentlicht in: | Analytical biochemistry 1992-09, Vol.205 (2), p.372-374 |
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| container_end_page | 374 |
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| container_issue | 2 |
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| container_title | Analytical biochemistry |
| container_volume | 205 |
| creator | Lowerson, Shelagh A. Taylor, Louise Briggs, Helen L. Turnbull, Douglass M. |
| description | We have developed methods for preparing a mitochondrial fraction from six to eight 80-cm super(2) flasks and optimizing the measurement of the activity of individual respiratory chain complexes. We have demonstrated that these techniques are sufficiently sensitive to detect respiratory chain defects in patients. |
| doi_str_mv | 10.1016/0003-2697(92)90453-E |
| format | Article |
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Miscellaneous investigative techniques</subject><subject>Proteins - analysis</subject><subject>respiration</subject><subject>Skin Physiological Phenomena</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EKtvCPwDJB4TKIcWOP3OphKqFIrXiAmfLsSdaoyRebKdi_z0Ouyo3epqvZ0Yz7yD0hpIrSqj8SAhhTSs7ddm1HzrCBWu2z9CGkk42hJHuOdo8Ii_Rec4_CaGUC3mGzijnTGi9QeEebF4STDAXHAdcdoCtK-EhlMMah9lX3y92xAnyPiRbYjpgt7Nhxi5O-xF-Q64YDjmOtoDHQ-hT7EebC55CiW4XZ5-CfYVeDHbM8PpkL9CPz9vvN7fN3bcvX28-3TWOU1UazzrdatkJJ4WWvIdOOyfYwLlb0wQ05bre4b1ViqtWqOpJoXo-OMIGxi7Q--PcfYq_FsjFTCE7GEc7Q1yyUYxRzjR_EqSS85YTWkF-BF2KOScYzD6FyaaDocSsrzCrzmbV2XSt-fsKs61tb0_zl34C_6_pqH2tvzvVbXZ2HJKdXciPmBCKCiIrdn3EoIr2ECCZ7ALMDnxI4IrxMfx_jz9LVKXE</recordid><startdate>19920901</startdate><enddate>19920901</enddate><creator>Lowerson, Shelagh A.</creator><creator>Taylor, Louise</creator><creator>Briggs, Helen L.</creator><creator>Turnbull, Douglass M.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19920901</creationdate><title>Measurement of the activity of individual respiratory chain complexes in isolated fibroblast mitochondria</title><author>Lowerson, Shelagh A. ; Taylor, Louise ; Briggs, Helen L. ; Turnbull, Douglass M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-d39828695c65864be98cc53f44c28690e8148011dda7747257dda657b4fc03f33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Biological and medical sciences</topic><topic>Citrate (si)-Synthase - analysis</topic><topic>Culture Techniques</topic><topic>Electron Transport - physiology</topic><topic>electron transport chain</topic><topic>fibroblasts</topic><topic>Fibroblasts - chemistry</topic><topic>Humans</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>man</topic><topic>measurements</topic><topic>Medical sciences</topic><topic>Metabolic diseases</topic><topic>methodology</topic><topic>mitochondria</topic><topic>Mitochondria - chemistry</topic><topic>Pathology. 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| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1992-09, Vol.205 (2), p.372-374 |
| issn | 0003-2697 1096-0309 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biological and medical sciences Citrate (si)-Synthase - analysis Culture Techniques Electron Transport - physiology electron transport chain fibroblasts Fibroblasts - chemistry Humans Investigative techniques, diagnostic techniques (general aspects) man measurements Medical sciences Metabolic diseases methodology mitochondria Mitochondria - chemistry Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proteins - analysis respiration Skin Physiological Phenomena |
| title | Measurement of the activity of individual respiratory chain complexes in isolated fibroblast mitochondria |
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