Measurement of the activity of individual respiratory chain complexes in isolated fibroblast mitochondria

We have developed methods for preparing a mitochondrial fraction from six to eight 80-cm super(2) flasks and optimizing the measurement of the activity of individual respiratory chain complexes. We have demonstrated that these techniques are sufficiently sensitive to detect respiratory chain defects...

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Veröffentlicht in:	Analytical biochemistry 1992-09, Vol.205 (2), p.372-374
Hauptverfasser:	Lowerson, Shelagh A., Taylor, Louise, Briggs, Helen L., Turnbull, Douglass M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Citrate (si)-Synthase - analysis Culture Techniques Electron Transport - physiology electron transport chain fibroblasts Fibroblasts - chemistry Humans Investigative techniques, diagnostic techniques (general aspects) man measurements Medical sciences Metabolic diseases methodology mitochondria Mitochondria - chemistry Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Proteins - analysis respiration Skin Physiological Phenomena
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Beschreibung
Zusammenfassung:	We have developed methods for preparing a mitochondrial fraction from six to eight 80-cm super(2) flasks and optimizing the measurement of the activity of individual respiratory chain complexes. We have demonstrated that these techniques are sufficiently sensitive to detect respiratory chain defects in patients.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(92)90453-E