Lion ( Panthera leo) and cheetah ( Acinonyx jubatus) IFN-γ sequences

Lion ( Panthera leo) and cheetah ( Acinonyx jubatus) IFN-γ sequences

Cloning and sequencing of the full length lion and cheetah interferon-gamma (IFN-γ) transcript will enable the expression of the recombinant cytokine, to be used for production of monoclonal antibodies and to set up lion and cheetah-specific IFN-γ ELISAs. These are relevant in blood-based diagnosis...

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Veröffentlicht in:Veterinary immunology and immunopathology 2010-04, Vol.134 (3), p.296-298
Hauptverfasser: Maas, Miriam, Van Rhijn, Ildiko, Allsopp, Maria T.E.P., Rutten, Victor P.M.G.
Format: Artikel
Sprache:eng
Schlagworte:
Acinonyx - genetics
Acinonyx - immunology
Acinonyx jubatus
Amino Acid Sequence
Animals
Antibodies, Monoclonal - biosynthesis
Base Sequence
Cats
Cattle
Cheetah
Cloning, Molecular
cytokines
DNA Primers - genetics
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - veterinary
Feline
IFN-γ
interferon-gamma
Interferon-gamma - genetics
interferons
Lion
Lions - genetics
Lions - immunology
molecular cloning
Molecular Sequence Data
monoclonal antibodies
Mycobacterium bovis
nucleotide sequences
Panthera leo
Sequence
sequence analysis
Sequence Homology, Amino Acid
South Africa
Species Specificity
Tuberculosis, Bovine - diagnosis
Tuberculosis, Bovine - transmission
wildlife
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Beschreibung
Zusammenfassung:Cloning and sequencing of the full length lion and cheetah interferon-gamma (IFN-γ) transcript will enable the expression of the recombinant cytokine, to be used for production of monoclonal antibodies and to set up lion and cheetah-specific IFN-γ ELISAs. These are relevant in blood-based diagnosis of bovine tuberculosis, an important threat to lions in the Kruger National Park. Alignment of nucleotide and amino acid sequences of lion and cheetah and that of domestic cats showed homologies of 97–100%.
ISSN:0165-2427
1873-2534
DOI:10.1016/j.vetimm.2009.10.001