DNA typing using AmpFlSTR Y-filer for very long-term stored specimens

Abstract Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22–30 years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis w...

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Veröffentlicht in:	Legal medicine (Tokyo, Japan) Japan), 2009-04, Vol.11, p.S466-S467
Hauptverfasser:	Tsukada, Kazuhiko, Shimizu, Maiko, Kurasawa, Yoshinobu, Kasahara, Koichi
Format:	Artikel
Sprache:	eng
Schlagworte:	AmpFlSTR Y-filer Blood Stains Chromosomes, Human, Y Degraded specimen DNA - isolation & purification DNA Degradation, Necrotic DNA Fingerprinting DNA typing Electrophoresis, Agar Gel Humans Internal Medicine Male Old bloodstain Polymerase Chain Reaction Specimen Handling Time Factors Y-STR
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Zusammenfassung:	Abstract Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22–30 years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis with an ABI 310 Genetic Analyzer and determined alleles using GeneMapper ID v3.2 software. Our results suggest that Y-filer finds certain loci more difficult to anneal than others.
ISSN:	1344-6223 1873-4162
DOI:	10.1016/j.legalmed.2009.01.057