An Old Method for Good New Cells

Abstract The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac...

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Veröffentlicht in:	Transplantation proceedings 2009-05, Vol.41 (4), p.1116-1118
Hauptverfasser:	Damiano, G, Gioviale, M.C, Lombardo, C, Lo Monte, A.I
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Allopurinol Animals Antigens, CD19 - metabolism Aorta, Abdominal Biological and medical sciences Cell Survival Female Ficoll Fundamental and applied biological sciences. Psychology Fundamental immunology Glutathione Insulin Male Medical sciences Organ Preservation Solutions Pancreas - cytology Perfusion - methods Raffinose Rats, Wistar Surgery Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases Tissue, organ and graft immunology
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Zusammenfassung:	Abstract The aim of this work was to demonstrate a greater number of viable cells using a micro-surgical in-situ perfusion to collect rat pancreata compared with the pancreas after exsanguination. We used 3 groups of 20 rats. Perfusion was performed by selective cannulation of the left common iliac artery with administration of UW solution at 4°C. Collected pancreata were digested and cells separated by Ficoll gradient were placed in culture to permit adhesion to dishes. Cells were characterized and tested for viability. We observed a gain of about 14% in the number of viable cells compared with those obtained after exsanguination ( P < .001 by chi-square).
ISSN:	0041-1345 1873-2623
DOI:	10.1016/j.transproceed.2009.03.078