A rapid chemiluminescent detection method for barley yellow dwarf virus

Barley yellow dwarf virus (BYDV-PAV-IL) was detected with biotinylated in vitro transcript cDNA using a chemiluminescent substrate on nylon membranes. Signals were detected on X-ray film and quantified using either a densitometer or an ELISA plate reader. The time required for sample preparation was...

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Veröffentlicht in:	Journal of virological methods 1992-09, Vol.39 (3), p.291-298
Hauptverfasser:	Fouly, Hanafy M., Domier, Leslie L., D'Arcy, Cleora J.
Format:	Artikel
Sprache:	eng
Schlagworte:	ADN AVENA BARLEY YELLOW DWARF VIRUS Biological and medical sciences CHEMILUMINESCENCE IMMUNOASSAYS DNA Dot blot ELISA Fundamental and applied biological sciences. Psychology Hordeum - microbiology Hybridization Luminescent Measurements Luteovirus Microbiology Plant Diseases - microbiology Plant Viruses - isolation & purification RNA Probes RNA, Viral - isolation & purification TECHNIQUE IMMUNOCHIMIOLUMINESCENTE TECNICAS INMUNOQUIMIOLUMINISCENTES TEST ELISA Virology VIRUS ENANISMO AMARILLO DE CEBADA VIRUS JAUNISSE NANISANTE DE L'ORGE
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Beschreibung
Zusammenfassung:	Barley yellow dwarf virus (BYDV-PAV-IL) was detected with biotinylated in vitro transcript cDNA using a chemiluminescent substrate on nylon membranes. Signals were detected on X-ray film and quantified using either a densitometer or an ELISA plate reader. The time required for sample preparation was reduced so that the entire protocol could be completed in two days. The in vitro transcript probes could detect 1 ng of purified virus and as little as 1 μl of sap extracts prepared from infected oat shoots.
ISSN:	0166-0934 1879-0984
DOI:	10.1016/0166-0934(92)90102-J