Secretion of alpha-hemolysin by bovine mammary isolates of Staphylococcus aureus

A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein hydrolysate and yeast extract in an atmosphere of 7% (v/v) CO2 in air. The assay consisted of a dot im...

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Veröffentlicht in:	Canadian journal of veterinary research 1992-07, Vol.56 (3), p.265-268
Hauptverfasser:	Kenny, K, Bastida, F.D, Norcross, N.L
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Toxins - biosynthesis Cattle dairy cows erythrocytes Female Hemolysin Proteins - biosynthesis hemolysins Hybridomas immunoblotting Life Sciences & Biomedicine mammary glands Mammary Glands, Animal - microbiology Mastitis, Bovine - microbiology Milk - microbiology Science & Technology Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - metabolism Staphylococcus aureus - pathogenicity strain differences Veterinary Sciences Virulence
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Zusammenfassung:	A total of 262 strains of Staphylococcus aureus isolated from the mammary gland of dairy cows were examined for the production of alpha-hemolysin. Strains were cultured in a liquid medium of casein hydrolysate and yeast extract in an atmosphere of 7% (v/v) CO2 in air. The assay consisted of a dot immunoblotting technique employing bacterial culture supernatants and a mouse monoclonal antibody specific for alpha-hemolysin. Ninety-four percent (247) of 262 strains were positive for alpha-hemolysin by this method, when cultured in the laboratory. This figure is compared with those obtained in previous studies which typically based their results on the hemolytic patterns of isolates on blood agar plates.
ISSN:	0830-9000