Effect of monensin on the sulfation of heparan sulfate proteoglycan from endothelial cells

Monensin is a monovalent metal ionphore that affects the intracellular translocation of secretory proteins at the level of trans‐Golgi cisternae. Exposure of endothelial cells to monensin results in the synthesis of heparan sulfate and chondroitin sulfate with a lower degree of sulfation. The inhibition it dose dependent and affects the ratio [35S]‐sulfate/[3H]‐hexosamine of heparan sulfate from both cells and medium, with no changes in their molecular wieght. By the use of several degradative enzymes (heparitinases, glycuronidase, and sulfatases) the fine structure of the heparan sulfate synthesized by control and monensin‐treated cells was investigated. The results have shown that among the six heparan sulfate disaccharides there is a specific decrease of the ones bearing a sulfate ester at the 6‐position of the glucosamine moiety. All other biosyntheitc steps were not affected by monensin. The results are indicative that monensin affects the hexosamine C‐6 sulfation, and that this sterification is the last step of the heparan sulfate biosynthesis and should occur at the trans‐Golgi compartment. © 1992 Wiley‐Liss, Inc.