Cloning of the gene coding for Staphylococcus intermedius exfoliative toxin and its expression in Escherichia coli

An exfoliative toxin (SIET)-producing strain (D-52) of Staphylococcus intermedius derived from canine pyoderma did not possess large plasmids. Therefore, the gene coding for SIET was considered to be located on the chromosomal DNA. The SIET gene was cloned from the chromosomal DNA of S. intermedius...

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Veröffentlicht in:	Veterinary microbiology 2003-06, Vol.94 (1), p.31-38
Hauptverfasser:	Terauchi, Rie, Sato, Hisaaki, Endo, You, Aizawa, Chikara, Maehara, Nobutoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Bacterial Toxins - biosynthesis Bacterial Toxins - genetics Bacteriology Base Sequence Biological and medical sciences Chickens DNA, Bacterial - genetics DNA, Bacterial - metabolism dogs Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Exfoliatins - biosynthesis Exfoliatins - genetics Fundamental and applied biological sciences. Psychology Gene genes Genes, Bacterial - genetics Microbiology molecular cloning Molecular Sequence Data Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains plasmids Polymerase Chain Reaction pyoderma Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Sequence Homology, Amino Acid signal peptide Staphylococcus - genetics Staphylococcus - metabolism Staphylococcus intermedius Toxins
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Zusammenfassung:	An exfoliative toxin (SIET)-producing strain (D-52) of Staphylococcus intermedius derived from canine pyoderma did not possess large plasmids. Therefore, the gene coding for SIET was considered to be located on the chromosomal DNA. The SIET gene was cloned from the chromosomal DNA of S. intermedius and was expressed in Escherichia coli. The nucleotide sequence of the SIET gene consists of a coding region of 990 bp specifying a polypeptide of 330 amino acid residues, which included a putative 42-residue signal sequence.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/S0378-1135(03)00047-6