NEW METHOD OF DETECTING NITRIC OXIDE PRODUCTION

Nitric oxide (NO) reacts with H2O2 to produce a rise in potent luminol-dependent chemiluminescence under neutral pH conditions. We applied this reaction to the continuous detection of NO released from the rat isolated perfused kidney with simultaneous pressure changes. Acetylcholine increased NO rel...

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Veröffentlicht in:	Chemical & pharmaceutical bulletin 1992/08/25, Vol.40(8), pp.2233-2235
Hauptverfasser:	KIKUCHI, Kazuya, HAYAKAWA, Hiroshi, NAGANO, Tetsuo, HIRATA, Yasunobu, SUGIMOTO, Tsuneaki, HIROBE, Masaaki
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences chemiluminescence Fundamental and applied biological sciences. Psychology In Vitro Techniques Kidney - metabolism luminol Male nitric oxide Nitric Oxide - analysis Nitric Oxide - metabolism perfused rat kidney Rats Rats, Wistar Vertebrates: urinary system
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Beschreibung
Zusammenfassung:	Nitric oxide (NO) reacts with H2O2 to produce a rise in potent luminol-dependent chemiluminescence under neutral pH conditions. We applied this reaction to the continuous detection of NO released from the rat isolated perfused kidney with simultaneous pressure changes. Acetylcholine increased NO release dose-dependently with the pressure reduction. An NO synthesis inhibitor, NG-monomethyl-L-arginine, abolished the release. This inhibition was reversed by the addition of L-arginine.
ISSN:	0009-2363 1347-5223
DOI:	10.1248/cpb.40.2233