A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

A metallothionein and CPx-ATPase handle heavy-metal tolerance in the filamentous cyanobacterium Oscillatoria brevis

A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn 2+ or Cd 2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn 2+ and Cd 2+ tolerance...

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Veröffentlicht in:FEBS letters 2003-05, Vol.542 (1), p.159-163
Hauptverfasser: Liu, Tong, Nakashima, Susumu, Hirose, Kazunobu, Uemura, Yoshiko, Shibasaka, Mineo, Katsuhara, Maki, Kasamo, Kunihiro
Format: Artikel
Sprache:eng
Schlagworte:
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - physiology
Amino Acid Sequence
Bacterial Proteins - genetics
Bacterial Proteins - physiology
bmtA gene
bxa1 gene
Cadmium - pharmacology
CPx-ATPase
Cyanobacteria - drug effects
Cyanobacteria - enzymology
Cyanobacteria - genetics
Cyanobacteria - metabolism
Cyanobacterium
Cyanophyta
Dose-Response Relationship, Drug
Escherichia coli - genetics
Gene Expression Regulation, Bacterial
Heavy metal tolerance
Kinetics
Metallothionein
Metallothionein - genetics
Metallothionein - physiology
Metals, Heavy - pharmacology
Molecular Sequence Data
Oscillatoria brevis
RNA, Messenger - biosynthesis
Sequence Homology, Amino Acid
Zinc - pharmacology
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Zusammenfassung:A metallothionein (BmtA) and a CPx-ATPase (Bxa1) have been identified and characterized from the cyanobacterium Oscillatoria brevis. Both bmtA and bxa1 expression can be markedly induced in vivo by Zn 2+ or Cd 2+. Over-expression of bmtA or bxa1 in Escherichia coli enhances Zn 2+ and Cd 2+ tolerance in the transformant. Dynamic studies on the expression of two genes showed that the maximum expression of bxa1 induced by Zn 2+ and Cd 2+ was much quicker than that of bmtA, suggesting distinct physiological roles of metallothionein and CPx-ATPase in the handling of surplus metal.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(03)00370-3