An Effective Concentration Method for Human Immunodeficiency Virus Type 1 (HIV-1)

We designed an effective virus concentration method to prevent the infection with human immunodeficiency virus type 1 (HIV-1) in laboratories. The absorbent of Minicon ® concentrators (Amicon Division, M.R. Grace & Co.-Conn.) was changed to chitin, a mucopolysaccharide extracted from the shells of Japanese pink crab. HIV-1 in the supernatant of HIV-1 infected Molt-4 cells was concentrated by Minicon ® and the new concentrators. The new concentrator showed good concentration rate and equality of concentration speed.