Vectors for generating nested deletions and facilitating subcloning G + C-rich DNA between Escherichia coli and Streptomyces sp

New multiple cloning sites (MCS), which facilitate the subcloning of G + C-rich DNA, were added to pUC18, M13mp18, pVE616 (a pBR322-derived insertion vector), and the low-copy-number Streptomyces vector, pIJ922. The MCS in these vectors contain sites found infrequently in Streptomyces DNA, facilitat...

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Veröffentlicht in:Gene 1992-09, Vol.119 (1), p.149-150
Hauptverfasser: Gewain, Keith M., Occi, James L., Foor, Forrest, MacNeil, Douglas J.
Format: Artikel
Sprache:eng
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Zusammenfassung:New multiple cloning sites (MCS), which facilitate the subcloning of G + C-rich DNA, were added to pUC18, M13mp18, pVE616 (a pBR322-derived insertion vector), and the low-copy-number Streptomyces vector, pIJ922. The MCS in these vectors contain sites found infrequently in Streptomyces DNA, facilitating the exchange of subclones between the vectors. The MCS added to M13mp18 and pUC18 was also designed to generate nested deletions within subcloned fragments.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(92)90083-2