A bioassay for cobra cardiotoxin activity using semi-isolated cockroach heart

A semi-isolated cockroach heart preparation was used to rapidly determine the activity of cobra cardiotoxin, monitored as a direct response on heart rate. This preparation produced a dose-response curve in the presence of active cardiotoxin and demonstrated that cardiotoxin retained its biological a...

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Veröffentlicht in:	Toxicon (Oxford) 1992-03, Vol.30 (3), p.295-301
Hauptverfasser:	Klowden, Marc J., Vitale, Angelo J., Trumble, Mayme J., Wesson, Carla Ruder, Trumble, William R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal poisons toxicology. Antivenoms Animals Biological and medical sciences Biological Assay Cobra Cardiotoxin Proteins - analysis Cobra Cardiotoxin Proteins - metabolism Cobra Cardiotoxin Proteins - pharmacology Cockroaches - drug effects Cross-Linking Reagents Heart Rate - drug effects Medical sciences Membrane Proteins - metabolism Molecular Weight Myocardium - metabolism Succinimides Toxicology
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Beschreibung
Zusammenfassung:	A semi-isolated cockroach heart preparation was used to rapidly determine the activity of cobra cardiotoxin, monitored as a direct response on heart rate. This preparation produced a dose-response curve in the presence of active cardiotoxin and demonstrated that cardiotoxin retained its biological activity after boiling, although cardiotoxin activity was destroyed by heating in the presence of dithiothreitol. Experiments that cross-linked radiolabeled cardiotoxin to solubilized cockroach heart membranes suggested that cardiotoxin bound specifically to a 59,000 mol. wt membrane protein in this tissue.
ISSN:	0041-0101 1879-3150
DOI:	10.1016/0041-0101(92)90870-B