Polypeptide composition of the 8S form of prolyl-tRNA synthetase from rat liver

Rat liver Fraction X containing the 24S complex of nine aminoacyl-tRNA synthetases, including prolyl-tRNA synthetase, was centrifuged on a 15–35% sucrose density gradient to obtain the 8S form of prolyl-tRNA synthetase. The enzyme was purified on a prolyldiaminohexyl-Sepharose 4B affinity column, sp...

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Veröffentlicht in:Biochemical and biophysical research communications 1992-09, Vol.187 (2), p.1071-1076
Hauptverfasser: Bianchi, Cristina, Perego, Roberto, Del Monte, Ugo
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Sprache:eng
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Zusammenfassung:Rat liver Fraction X containing the 24S complex of nine aminoacyl-tRNA synthetases, including prolyl-tRNA synthetase, was centrifuged on a 15–35% sucrose density gradient to obtain the 8S form of prolyl-tRNA synthetase. The enzyme was purified on a prolyldiaminohexyl-Sepharose 4B affinity column, specifically binding prolyl-tRNA synthetase to Sepharose-bound proline. After SDS-polyacrylamide gel electrophoresis, two peptides of 58 and 61 kDa were detected in the peak of prolyl-tRNA synthetase activity eluted from the affinity column. The 58 and 61 kDa peptides were also present in the 24S complex containing prolyl-tRNA synthetase activity isolated on the sucrose density gradient.
ISSN:0006-291X
1090-2104
DOI:10.1016/0006-291X(92)91306-B