Genomic Organization and Promoter Analysis of Mouse Apoptosis Signal-regulating Kinase 2 (ASK 2)
ASK 2/MAPKKK 6 was identified as a member of the MAPKKK family, which shares 45% amino acid identity with ASK 1/MAPKKK 5. In this study, the genomic organization and promoter of mouse ASK 2 were determined. ASK 2 gene spans approximately 12 kb and is comprised of 29 exons, flanked by 5' regulat...
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Veröffentlicht in: | Kōkūbyō Gakkai Zasshi JAPAN, 2003/03/31, Vol.70(1), pp.9-18 |
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Sprache: | jpn |
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Zusammenfassung: | ASK 2/MAPKKK 6 was identified as a member of the MAPKKK family, which shares 45% amino acid identity with ASK 1/MAPKKK 5. In this study, the genomic organization and promoter of mouse ASK 2 were determined. ASK 2 gene spans approximately 12 kb and is comprised of 29 exons, flanked by 5' regulatory regions. Through comparison with ASK 2 cDNA variant clones, two splice variants were identified : a transcript lacking 24 bases encoding 8 amino acids was caused by disuse of exon 3, and another frameshifted transcript due to 4 bases deletion was produced by usage of the first 4 bases of exon 17 as a part of the splicing acceptor. Reporter plasmid analysis demonstrated that a promoter existed in the immediate upstream region adjacent to exon 1. Interestingly, the 5'-flanking region of the promoter possessed a strong suppressive element. These results suggest that expression of ASK 2 is regulated by multiple mechanisms. |
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ISSN: | 0300-9149 1884-5185 |
DOI: | 10.5357/koubyou.70.9 |