Genomic organization and full-length cDNA sequence of human collagen X

We have determined the full-length cDNA sequence of the human αl(X) collagen gene by sequence analysis of a genomic clone ERG [(1991) Dev. Biol. 148, 562–572], and of cDNA fragments generated from a reverse transcribed as αl(X) mRNA by PCR. We defined the promoter region, the transcription initiation site and the full-length 5′-untranslated region. We also report the exon/intron boundaries of the transcript and the complete 3′-untranslated region as well as a 3′-flanking sequence containing two additional polyadenylation signals. The promoter region is homologous to chicken and mouse type X promoters within several highly conserved regions. The genomic organization shows high homologies to chicken and mouse.