Activated human T cells express a ligand for the human B cell‐associated antigen CD40 which participates in T cell‐dependent activation of B lymphocytes

To identify the ligand for the B cell‐associated antigen CD40, we constructed a chimeric immunoglobulin molecule where the extracellular portion of the CD40 protein replaced the normal immunoglobulin variable region. No binding was detected on resting peripheral blood T cells. However, following T c...

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Veröffentlicht in:European journal of immunology 1992-10, Vol.22 (10), p.2573-2578
Hauptverfasser: Lane, Peter, Traunecker, André, Hubele, Sabine, Inui, Seiji, Lanzavecchia, Antonio, Gray, David
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Sprache:eng
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Zusammenfassung:To identify the ligand for the B cell‐associated antigen CD40, we constructed a chimeric immunoglobulin molecule where the extracellular portion of the CD40 protein replaced the normal immunoglobulin variable region. No binding was detected on resting peripheral blood T cells. However, following T cell activation with phorbol esters and ionomycin, the chimeric protein bound specifically to activated human T cells and precipitated a 35‐kDa protein from such cells. The induction of the CD40 ligand was detectable on the cell surface after 1 h, with maximal expression after 8 h of stimulation. The T cells expressing CD40 ligand were predominantly CD4 positive, although a proportion of CD8‐positive cells also expressed the protein. There was no particular correlation with CD45 phenotype. Finally, we found that soluble CD40 inhibited T‐dependent B cell proliferation. The results are discussed in the context of cognate interactions between B and T cells.
ISSN:0014-2980
1521-4141
DOI:10.1002/eji.1830221016