Determination of ampicillin in the presence of cloxacillin

A spectrophotometric method is described for the assay of ampicillin in the presence of cloxacillin in pharmaceutical preparations. It involves the reaction of hydrolysed ampicillin with formaldehyde in an acidic phosphate buffer (pH 2.5) and measurement of the absorbance of the product at 373 nm af...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of pharmaceutical and biomedical analysis 1992, Vol.10 (1), p.43-47
Hauptverfasser:	Akanni, A.O., Ayim, J.S.K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Ampicillin Ampicillin - analysis Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences capsules cloxacillin Cloxacillin - analysis combined preparations Dosage Forms Hydrogen-Ion Concentration injections Medical sciences Pharmacology. Drug treatments Spectrophotometry syrups Temperature
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A spectrophotometric method is described for the assay of ampicillin in the presence of cloxacillin in pharmaceutical preparations. It involves the reaction of hydrolysed ampicillin with formaldehyde in an acidic phosphate buffer (pH 2.5) and measurement of the absorbance of the product at 373 nm after dilution with 2 M sulphuric acid. The method is selective for ampicillin in the presence of cloxacillin. The absorbance had a linear relationship with concentration for the range studied (5–35 μg ml −1). By this method the ampicillin content of combined preparations of ampicillin and cloxacillin has been successfully determined in capsules, injections and a syrup.
ISSN:	0731-7085 1873-264X
DOI:	10.1016/0731-7085(92)80009-C