Physiologic weight-bearing increases new vessel formation during distraction osteogenesis: a micro-tomographic imaging study
During distraction osteogenesis, large volumes of new bone are formed through the slow distraction of fracture callus. The newly formed bone is closely linked to angiogenesis and positively influenced by physiologic loading. In this study, a rat model was used to explore the correlation between thes...
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| Veröffentlicht in: | Journal of orthopaedic research 2003-05, Vol.21 (3), p.489-496 |
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| creator | Moore, Douglas C Leblanc, Christopher W Müller, Ralph Crisco, Joseph J Ehrlich, Michael G |
| description | During distraction osteogenesis, large volumes of new bone are formed through the slow distraction of fracture callus. The newly formed bone is closely linked to angiogenesis and positively influenced by physiologic loading. In this study, a rat model was used to explore the correlation between these two observations. Unilateral femoral lengthenings were performed in 18 male Sprague–Dawley rats (400–500 g, age |
| doi_str_mv | 10.1016/S0736-0266(02)00234-6 |
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1, which included vessels in the medullary canal, cortex, and periosteum, and VOI
2, which included only vessels in the canal and cortex, the total tissue volume (TV), the volume of vessels (VV), and vessel diameter (VD) were determined. For the larger subvolume (VOI
1), VV and vessel density (VV/TV) increased as a function of time (
p<0.001) in WB animals. In NWB animals, VV increased significantly with time (
p=0.029), while VV/TV did not (
p=0.36). Increases in VV and VV/TV were significantly greater in WB animals than in NWB animals (
p<0.01 and 0.05, respectively). VD was similar in both groups and did not change with time. Our data suggest that weight bearing stimulates new vessel formation during distraction osteogenesis.</description><identifier>ISSN: 0736-0266</identifier><identifier>EISSN: 1554-527X</identifier><identifier>DOI: 10.1016/S0736-0266(02)00234-6</identifier><identifier>PMID: 12706022</identifier><identifier>CODEN: JOREDR</identifier><language>eng</language><publisher>Hoboken: Elsevier Ltd</publisher><subject>Angiogenesis ; Animals ; Bone lengthening ; Distraction osteogenesis ; Femur - diagnostic imaging ; Femur - physiology ; Femur - surgery ; Image Processing, Computer-Assisted ; Male ; Neovascularization, Physiologic - physiology ; Osteogenesis - physiology ; Osteogenesis, Distraction ; Physical stimulation ; Rats ; Rats, Sprague-Dawley ; Tomography, X-Ray Computed ; Weight-Bearing - physiology</subject><ispartof>Journal of orthopaedic research, 2003-05, Vol.21 (3), p.489-496</ispartof><rights>2003 Orthopaedic Research Society</rights><rights>Copyright © 2003 Orthopaedic Research Society</rights><rights>Copyright Journal of Bone and Joint Surgery, Inc. 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c6326-64b55f5ad36b162dd87e6f9885bc58a3ed9a45ad59478182736ad88904ae931c3</citedby><cites>FETCH-LOGICAL-c6326-64b55f5ad36b162dd87e6f9885bc58a3ed9a45ad59478182736ad88904ae931c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2FS0736-0266%2802%2900234-6$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2FS0736-0266%2802%2900234-6$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,1428,27905,27906,45555,45556,46390,46814</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12706022$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Moore, Douglas C</creatorcontrib><creatorcontrib>Leblanc, Christopher W</creatorcontrib><creatorcontrib>Müller, Ralph</creatorcontrib><creatorcontrib>Crisco, Joseph J</creatorcontrib><creatorcontrib>Ehrlich, Michael G</creatorcontrib><title>Physiologic weight-bearing increases new vessel formation during distraction osteogenesis: a micro-tomographic imaging study</title><title>Journal of orthopaedic research</title><addtitle>J. Orthop. Res</addtitle><description>During distraction osteogenesis, large volumes of new bone are formed through the slow distraction of fracture callus. The newly formed bone is closely linked to angiogenesis and positively influenced by physiologic loading. In this study, a rat model was used to explore the correlation between these two observations. Unilateral femoral lengthenings were performed in 18 male Sprague–Dawley rats (400–500 g, age<6 months). Half of the animals were allowed to bear weight freely (WB) while the remaining animals were made non-weight-bearing via a through-knee amputation (NWB). After a seven-day latency period, femurs were lengthened 7 mm over 21 days. Animals were sacrificed at 7, 21, 35, and 49 days (0, 4.7, 7, and 7 mm of distraction) at which time lower extremity vessels were perfused with a 60% (w/v) barium sulfate suspension. High-resolution three-dimensional images of the vascular architecture were generated using a fan-beam micro-tomography machine by digitally separating the contrast-filled vessels from surrounding tissue. For two subvolumes, VOI
1, which included vessels in the medullary canal, cortex, and periosteum, and VOI
2, which included only vessels in the canal and cortex, the total tissue volume (TV), the volume of vessels (VV), and vessel diameter (VD) were determined. For the larger subvolume (VOI
1), VV and vessel density (VV/TV) increased as a function of time (
p<0.001) in WB animals. In NWB animals, VV increased significantly with time (
p=0.029), while VV/TV did not (
p=0.36). Increases in VV and VV/TV were significantly greater in WB animals than in NWB animals (
p<0.01 and 0.05, respectively). VD was similar in both groups and did not change with time. Our data suggest that weight bearing stimulates new vessel formation during distraction osteogenesis.</description><subject>Angiogenesis</subject><subject>Animals</subject><subject>Bone lengthening</subject><subject>Distraction osteogenesis</subject><subject>Femur - diagnostic imaging</subject><subject>Femur - physiology</subject><subject>Femur - surgery</subject><subject>Image Processing, Computer-Assisted</subject><subject>Male</subject><subject>Neovascularization, Physiologic - physiology</subject><subject>Osteogenesis - physiology</subject><subject>Osteogenesis, Distraction</subject><subject>Physical stimulation</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Tomography, X-Ray Computed</subject><subject>Weight-Bearing - physiology</subject><issn>0736-0266</issn><issn>1554-527X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkc1u1DAUhSMEokPhEUARCwSLgH9iO2EDqNACGrVQQCA2lmPfZFySeLCTDiPx8DiTUZHYlI0tX3_3XB-fJLmP0VOMMH_2CQnKM0Q4f4zIE4QIzTN-I1lgxvKMEfHtZrK4Qg6SOyFcIIQEJsXt5AATgTgiZJH8_rDaButa11idbsA2qyGrQHnbN6nttQcVIKQ9bNJLCAHatHa-U4N1fWrGHWVsGLzSu5ILA7gGegg2PE9V2lntXTa4zjVerVdxhO1UM3WFYTTbu8mtWrUB7u33w-TL8ZvPR2-z5dnJu6NXy0xzSnjG84qxmilDeYU5MaYQwOuyKFilWaEomFLl8ZqVuShwQaJrZYqiRLmCkmJND5NHs-7au58jhEF2NmhoW9WDG4MUlCDOGb8WxIXIKSdlBB_-A1640ffRhCSUYVSSHEeIzVD8hBA81HLto3-_lRjJKUS5C1FOCcVF7kKU0yse7MXHqgPzt2ufWgRezsDGtrD9P1X5_uwc43jAiOJpRjZLxPTg15WE8j8kF1Qw-fX0RB4vP4rv56elfB35FzMPMadLC14GbaHXYKwHPUjj7DWu_gAh9c70</recordid><startdate>200305</startdate><enddate>200305</enddate><creator>Moore, Douglas C</creator><creator>Leblanc, Christopher W</creator><creator>Müller, Ralph</creator><creator>Crisco, Joseph J</creator><creator>Ehrlich, Michael G</creator><general>Elsevier Ltd</general><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200305</creationdate><title>Physiologic weight-bearing increases new vessel formation during distraction osteogenesis: a micro-tomographic imaging study</title><author>Moore, Douglas C ; Leblanc, Christopher W ; Müller, Ralph ; Crisco, Joseph J ; Ehrlich, Michael G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c6326-64b55f5ad36b162dd87e6f9885bc58a3ed9a45ad59478182736ad88904ae931c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Angiogenesis</topic><topic>Animals</topic><topic>Bone lengthening</topic><topic>Distraction osteogenesis</topic><topic>Femur - diagnostic imaging</topic><topic>Femur - physiology</topic><topic>Femur - surgery</topic><topic>Image Processing, Computer-Assisted</topic><topic>Male</topic><topic>Neovascularization, Physiologic - physiology</topic><topic>Osteogenesis - physiology</topic><topic>Osteogenesis, Distraction</topic><topic>Physical stimulation</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Tomography, X-Ray Computed</topic><topic>Weight-Bearing - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Moore, Douglas C</creatorcontrib><creatorcontrib>Leblanc, Christopher W</creatorcontrib><creatorcontrib>Müller, Ralph</creatorcontrib><creatorcontrib>Crisco, Joseph J</creatorcontrib><creatorcontrib>Ehrlich, Michael G</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of orthopaedic research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Moore, Douglas C</au><au>Leblanc, Christopher W</au><au>Müller, Ralph</au><au>Crisco, Joseph J</au><au>Ehrlich, Michael G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiologic weight-bearing increases new vessel formation during distraction osteogenesis: a micro-tomographic imaging study</atitle><jtitle>Journal of orthopaedic research</jtitle><addtitle>J. Orthop. Res</addtitle><date>2003-05</date><risdate>2003</risdate><volume>21</volume><issue>3</issue><spage>489</spage><epage>496</epage><pages>489-496</pages><issn>0736-0266</issn><eissn>1554-527X</eissn><coden>JOREDR</coden><abstract>During distraction osteogenesis, large volumes of new bone are formed through the slow distraction of fracture callus. The newly formed bone is closely linked to angiogenesis and positively influenced by physiologic loading. In this study, a rat model was used to explore the correlation between these two observations. Unilateral femoral lengthenings were performed in 18 male Sprague–Dawley rats (400–500 g, age<6 months). Half of the animals were allowed to bear weight freely (WB) while the remaining animals were made non-weight-bearing via a through-knee amputation (NWB). After a seven-day latency period, femurs were lengthened 7 mm over 21 days. Animals were sacrificed at 7, 21, 35, and 49 days (0, 4.7, 7, and 7 mm of distraction) at which time lower extremity vessels were perfused with a 60% (w/v) barium sulfate suspension. High-resolution three-dimensional images of the vascular architecture were generated using a fan-beam micro-tomography machine by digitally separating the contrast-filled vessels from surrounding tissue. For two subvolumes, VOI
1, which included vessels in the medullary canal, cortex, and periosteum, and VOI
2, which included only vessels in the canal and cortex, the total tissue volume (TV), the volume of vessels (VV), and vessel diameter (VD) were determined. For the larger subvolume (VOI
1), VV and vessel density (VV/TV) increased as a function of time (
p<0.001) in WB animals. In NWB animals, VV increased significantly with time (
p=0.029), while VV/TV did not (
p=0.36). Increases in VV and VV/TV were significantly greater in WB animals than in NWB animals (
p<0.01 and 0.05, respectively). VD was similar in both groups and did not change with time. Our data suggest that weight bearing stimulates new vessel formation during distraction osteogenesis.</abstract><cop>Hoboken</cop><pub>Elsevier Ltd</pub><pmid>12706022</pmid><doi>10.1016/S0736-0266(02)00234-6</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of orthopaedic research, 2003-05, Vol.21 (3), p.489-496 |
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| language | eng |
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| subjects | Angiogenesis Animals Bone lengthening Distraction osteogenesis Femur - diagnostic imaging Femur - physiology Femur - surgery Image Processing, Computer-Assisted Male Neovascularization, Physiologic - physiology Osteogenesis - physiology Osteogenesis, Distraction Physical stimulation Rats Rats, Sprague-Dawley Tomography, X-Ray Computed Weight-Bearing - physiology |
| title | Physiologic weight-bearing increases new vessel formation during distraction osteogenesis: a micro-tomographic imaging study |
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