Desiccation Tolerance of Spermatozoa Dried at Ambient Temperature: Production of Fetal Mice

Long-term preservation of mouse sperm by desiccation is economically and logistically attractive. The current investigation is a feasibility study of the preservation of mouse sperm by convective drying in an inert gas (nitrogen). Mouse sperm from the B6D2F1 strain isolated in an EGTA-supplemented Tris-HCl buffer were dried using three different drying rates and were stored for 18–24 h at 4°C. The mean final moisture content was