Hypertonic stress induces αB-crystallin expression

AlphaB-crystallin, a major lens protein, was induced in primary cultures of dog lens epithelial cells and glomerular endothelial cells when they were grown under conditions of hypertonic stress. With Western blot analysis using a specific αB-crystallin antibody, we observed a significant increase in...

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Veröffentlicht in:Experimental eye research 1992-03, Vol.54 (3), p.461-470
Hauptverfasser: Dasgupta, Sarmila, Hohman, Thomas C., Carper, Deborah
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Sprache:eng
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Zusammenfassung:AlphaB-crystallin, a major lens protein, was induced in primary cultures of dog lens epithelial cells and glomerular endothelial cells when they were grown under conditions of hypertonic stress. With Western blot analysis using a specific αB-crystallin antibody, we observed a significant increase in the concentration of αB-crystallin protein in cells grown for 4–6 days in media supplemented with 150 m m NaCl or 250 m m cellobiose. These supplements increased the osmolarity of the medium from 300 to 550–600 mosmol kg −1. AlphaB-crystallin mRNA was also increased reaching a maximum four-fold increase in lens and 16-fold increase in kidney cells within 1–2 days. These studies demonstrate a type of regulation of αB-crystallin expression in cells from lenticular and non-lenticular tissues.
ISSN:0014-4835
1096-0007
DOI:10.1016/0014-4835(92)90058-Z