DNA extraction from mixtures of body fluid using mild preferential lysis

DNA extraction from mixtures of body fluid using mild preferential lysis

A modification to the DNA extraction method "preferential lysis" (Gill et al. 1985) is proposed which can be applied to DNA mixtures of vaginal cells and spermatozoa. In mixtures with a low sperm content the further loss of sperm DNA caused by the extraction can be avoided by using "m...

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Veröffentlicht in:International journal of legal medicine 1992-11, Vol.104 (6), p.359-360
Hauptverfasser: Wiegand, P, Schürenkamp, M, Schütte, U
Format: Artikel
Sprache:eng
Schlagworte:
Body Fluids - chemistry
Cervix Mucus - chemistry
DNA - analysis
DNA - genetics
Female
Humans
Male
Polymerase Chain Reaction - methods
Vaginal Smears
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Zusammenfassung:A modification to the DNA extraction method "preferential lysis" (Gill et al. 1985) is proposed which can be applied to DNA mixtures of vaginal cells and spermatozoa. In mixtures with a low sperm content the further loss of sperm DNA caused by the extraction can be avoided by using "mild preferential lysis". Amplification by PCR (polymerase chain reaction) then yields sufficient DNA to be able to identify both components in the mixture.
ISSN:0937-9827
1437-1596
DOI:10.1007/bf01369558