Technical note: Specific PCR amplification of protozoal 18S rDNA sequences from DNA extracted from ruminal samples of cows

A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-34...

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Veröffentlicht in:	Journal of animal science 2003-03, Vol.81 (3), p.812-815
Hauptverfasser:	Karnati, S.K.R, Yu, Z, Sylvester, J.T, Dehority, B.A, Morrison, M, Firkins, J.L
Format:	Artikel
Sprache:	eng
Schlagworte:	alfalfa hay Animals Base Sequence Cattle cows DNA Primers DNA, Protozoan - genetics DNA, Ribosomal - genetics Eukaryota - classification Eukaryota - genetics Gene Amplification Molecular Sequence Data nucleotide sequences Phylogeny polymerase chain reaction Polymerase Chain Reaction - veterinary ribosomal DNA ribosomal RNA RNA, Ribosomal, 18S - genetics Rumen - parasitology rumen protozoa Sequence Alignment - veterinary
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Beschreibung
Zusammenfassung:	A protozoa-specific primer (P-SSU-342f) was designed and paired with a eukarya-specific primer to amplify a 1,360-bp fragment of DNA encoding protozoal small subunit (SSU) ribosomal RNA from ruminal fluid of cows fed a mixed forage:grain diet or alfalfa hay. Sequencing of clones showed that P-SSU-342f is specific to ruminal protozoa and, with slight modifications, the primer will be useful for ecological studies of ruminal protozoa.
ISSN:	0021-8812 1525-3163