Expression of Matrix Metalloproteinases (MMPs) and Tissue Inhibitors of Metalloproteinases (TIMPs) in Healthy and Diseased Human Gingiva

Background: The purpose of this study was to quantify the amount of matrix metalloproteinases such as MMP‐1, MMP‐2, MMP‐3, MMP‐7, MMP‐9, and MMP‐13 and tissue inhibitors of metalloproteinases TIMP‐1 and TIMP‐2 expressed by human gingival explants in culture media and the area fraction (AA%) of gingival collagen fibers according to the degree of inflammation, to investigate a possible correlation between these enzymes and collagen loss. Methods: Gingival tissue specimens from 6 healthy controls (group 1), 17 patients with mild gingival inflammation (group 2), 10 patients with moderate gingival inflammation (group 3), and 9 patients with severe gingival inflammation (group 4) were placed in organ culture for 3 days. The MMPs and TIMPs in the culture media were quantified using zymography, dot blotting, and Western blotting. Paraffin gingival sections were stained with sirius red F3Ba for visualization of collagen fibers, then the area fraction (AA%) occupied by the gingival fibers was determined by automated image analysis. Results: The AA% occupied by collagen fibers significantly decreased from group 1 (53%) to group 4 (35%). The decrease in collagen fibers was inversely correlated with the significant increase in MMP‐1, MMP‐9, and MMP‐13 (dot blotting analysis), with the increase of the active form of MMP‐2, and with the active form and proform of MMP‐9 (zymography analysis). Conclusion: The present study showed that metalloproteinases, particularly MMP‐2, MMP‐9, MMP‐1, and MMP‐13, are involved in the gingival extracellular matrix degradation during periodontitis. J Periodontol 2003;74:188‐195.