Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea

1 Korean Agricultural Culture Collection (KACC), Genetic Resources Division, National Institute of Agricultural Biotechnology, 225th, Seodun-dong Gwonseon-gu, Suwon 441-707, Korea 2 Korean Agricultural Culture Collection (KACC), Metabolic Engineering Division, National Institute of Agricultural Biotechnology, 225th, Seodun-dong Gwonseon-gu, Suwon 441-707, Korea 4 Korean Agricultural Culture Collection (KACC), Molecular Physiology Division, National Institute of Agricultural Biotechnology, 225th, Seodun-dong Gwonseon-gu, Suwon 441-707, Korea 3 College of Agriculture and Life Sciences, 192-1, Hyoja2-Dong, Chunchon 200-701, Korea Correspondence Soon Wo Kwon swkwon{at}rda.go.kr Among Pseudomonas strains isolated from Korean agricultural soils, four strains (Ps 9-14 group: Ps 1-2, Ps 1-10, Ps 5-5 and Ps 9-14 T ) from the Suwon, Goesan and Samchok regions, three strains (Ps 3-10 group: Ps 2-22, Ps 3-1 and Ps 3-10 T ) from Umsong Region and four strains (Pss 26 group: Pss 14, Pss 25, Pss 26 T and Pss 27) from Jinju Region were identified as three independent groups on the basis of 16S rDNA sequence analysis. While, on the basis of 16S rDNA sequence analysis, Ps 9-14 T and Ps 3-10 T form a phyletic line with Pseudomonas jessenii CIP 105274 T , ‘ Pseudomonas pavonaceae ’ IAM 1155 and Pseudomonas graminis DSM 11363 T , Pss 26 T is grouped with Pseudomonas citronellolis ATCC 13674 T and Pseudomonas nitroreducens IAM 1439 T . According to DNA–DNA hybridization studies, strain Ps 9-14 T shows high DNA relatedness to strain Ps 3-10 T (52 %) and Pseudomonas migulae CIP 105470 T (49 %) and strain Ps 3-10 T reveals high relatedness to strain Ps 9-14 T (48 %) and P. jessenii CIP 105274 T (45 %). Strain Pss 26 T shows high relatedness to P. citronellolis LMG 18378 T (54 %), P. nitroreducens ATCC 33634 T (48 %) and Pseudomonas aeruginosa LMG 1242 T (48 %). On the basis of phenotypic and genotypic analyses, three novel species of the genus Pseudomonas are proposed: Pseudomonas koreensis sp. nov. (type strain Ps 9-14 T =LMG 21318 T =KACC 10848 T ) for the Ps 9-14 group, Pseudomonas umsongensis sp. nov. (type strain Ps 3-10 T =LMG 21317 T =KACC 10847 T ) for the Ps 3-10 group and Pseudomonas jinjuensis sp. nov. (type strain Pss 26 T =LMG 21316 T =KACC 10760 T ) for the Pss 26 group. Abbreviations: PAF, Pseudomonas agar F; TSA, trypticase soy agar The GenBank accession numbers for the 16S rDNA sequences described in this work are AF468448 – AF468453 , as indicated in Fig. 2. A 16