Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity
The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH) 2D 3. Cell v...
Gespeichert in:
| Veröffentlicht in: | Biomedicine & pharmacotherapy 2003, Vol.57 (1), p.57-62 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 62 |
|---|---|
| container_issue | 1 |
| container_start_page | 57 |
| container_title | Biomedicine & pharmacotherapy |
| container_volume | 57 |
| creator | Torricelli, P. Fini, M. Giavaresi, G. Borsari, V. Carpi, A. Nicolini, A. Giardino, R. |
| description | The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH)
2D
3. Cell viability was quite similar for primary osteoblast cultures (MTT: 1.64–2.11 OD); a significant (
P |
| doi_str_mv | 10.1016/S0753-3322(02)00329-3 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_73117857</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0753332202003293</els_id><sourcerecordid>73117857</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-a37276e3df91568902b416c179575151d85093a6de9f0ea6d26e0f6a8323c9d03</originalsourceid><addsrcrecordid>eNqFkVFrHCEQgCW0JJdLf0LKvjS0D9uOuuqal1CONC0E8pD0WTydbS17u1d1D-7f180dzWNgYND5HMdPQi4pfKZA5ZdHUILXnDP2EdgnAM50zU_IgmoBtQRQb8jiP3JGzlP6AwBC8vaUnFEmGwa8XZB-NW62NtocdliFIWNMW3QBU1nsMOXwq5TGoZojZRzXvU25clOfp4jpuvI227nosO-rXbDr0Ie8r-zgq7Qf8m_MwVXWlfZl-4K87Wyf8N0xL8nPb7dPq-_1_cPdj9XX-9o1Dc-15Yopidx3mgrZamDrhkpHlRZKUEF9K0BzKz3qDrBkJhE6aVvOuNMe-JJcHfpu4_h3Kq8wm5DmCe2A45SM4pSqVqgCigPo4phSxM5sY9jYuDcUzKzZPGs2s0MDJWbNZbUk748XTOsN-pdTR68F-HAEbHK276IdXEgvXFN-SAtRuJsDh0XHLmA0qcgfHPoQ0WXjx_DKKP8AlSWa0Q</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>73117857</pqid></control><display><type>article</type><title>Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Torricelli, P. ; Fini, M. ; Giavaresi, G. ; Borsari, V. ; Carpi, A. ; Nicolini, A. ; Giardino, R.</creator><creatorcontrib>Torricelli, P. ; Fini, M. ; Giavaresi, G. ; Borsari, V. ; Carpi, A. ; Nicolini, A. ; Giardino, R.</creatorcontrib><description>The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH)
2D
3. Cell viability was quite similar for primary osteoblast cultures (MTT: 1.64–2.11 OD); a significant (
P < 0.005) difference was found between sheep osteoblasts and MG-63 (ΔMTT: 0.52 ± 0.20 OD). Osteocalcin synthesis ranged from 15.18 to 27.00 pg/ml in primary osteoblast cultures, while it was significantly (
P < 0.01) lower in MG-63 (OC: 6.67 ± 0.52 pg/ml) when compared with primary human osteoblasts. Alkaline phosphatase, C-terminal procollagen type I, and interleukin-6 were significantly (
P < 0.005) lower in rat osteoblasts when compared with primary human osteoblasts, and similarly transforming growth factor-β1 was significantly (
P < 0.05) lower in rat and sheep osteoblasts when compared with primary human osteoblasts and MG-63. Nitric oxide synthesis did not show any significant difference either before or after vitamin 1,25(OH)
2D
3 stimulation. In conclusion, the current findings confirm the presence of interspecies differences between the selected osteoblast lineages before and after stimulation with vitamin1,25(OH)
2D
3. Above all, the culture of sheep osteoblasts was seen to behave more similarly to that of primary human cells, mainly in terms of cell viability, osteocalcin, interleukin-6 and transforming growth factor-β1 production.</description><identifier>ISSN: 0753-3322</identifier><identifier>EISSN: 1950-6007</identifier><identifier>DOI: 10.1016/S0753-3322(02)00329-3</identifier><identifier>PMID: 12642038</identifier><identifier>CODEN: BIPHEX</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Animals ; Biological and medical sciences ; Calcitriol - pharmacology ; Cell Differentiation - drug effects ; Cell Differentiation - physiology ; Cell Survival - drug effects ; Cell Survival - physiology ; Cells, Cultured ; Data Interpretation, Statistical ; Fundamental and applied biological sciences. Psychology ; Humans ; Osteoblasts ; Osteoblasts - cytology ; Osteoblasts - drug effects ; Osteoblasts - metabolism ; Rats ; Sheep ; Skeleton and joints ; Tumor Cells, Cultured - drug effects ; Tumor Cells, Cultured - metabolism ; Vertebrates: osteoarticular system, musculoskeletal system</subject><ispartof>Biomedicine & pharmacotherapy, 2003, Vol.57 (1), p.57-62</ispartof><rights>2003 Éditions scientifiques et médicales Elsevier SAS</rights><rights>2003 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-a37276e3df91568902b416c179575151d85093a6de9f0ea6d26e0f6a8323c9d03</citedby><cites>FETCH-LOGICAL-c443t-a37276e3df91568902b416c179575151d85093a6de9f0ea6d26e0f6a8323c9d03</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0753332202003293$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,4010,27902,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14600955$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12642038$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Torricelli, P.</creatorcontrib><creatorcontrib>Fini, M.</creatorcontrib><creatorcontrib>Giavaresi, G.</creatorcontrib><creatorcontrib>Borsari, V.</creatorcontrib><creatorcontrib>Carpi, A.</creatorcontrib><creatorcontrib>Nicolini, A.</creatorcontrib><creatorcontrib>Giardino, R.</creatorcontrib><title>Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity</title><title>Biomedicine & pharmacotherapy</title><addtitle>Biomed Pharmacother</addtitle><description>The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH)
2D
3. Cell viability was quite similar for primary osteoblast cultures (MTT: 1.64–2.11 OD); a significant (
P < 0.005) difference was found between sheep osteoblasts and MG-63 (ΔMTT: 0.52 ± 0.20 OD). Osteocalcin synthesis ranged from 15.18 to 27.00 pg/ml in primary osteoblast cultures, while it was significantly (
P < 0.01) lower in MG-63 (OC: 6.67 ± 0.52 pg/ml) when compared with primary human osteoblasts. Alkaline phosphatase, C-terminal procollagen type I, and interleukin-6 were significantly (
P < 0.005) lower in rat osteoblasts when compared with primary human osteoblasts, and similarly transforming growth factor-β1 was significantly (
P < 0.05) lower in rat and sheep osteoblasts when compared with primary human osteoblasts and MG-63. Nitric oxide synthesis did not show any significant difference either before or after vitamin 1,25(OH)
2D
3 stimulation. In conclusion, the current findings confirm the presence of interspecies differences between the selected osteoblast lineages before and after stimulation with vitamin1,25(OH)
2D
3. Above all, the culture of sheep osteoblasts was seen to behave more similarly to that of primary human cells, mainly in terms of cell viability, osteocalcin, interleukin-6 and transforming growth factor-β1 production.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Calcitriol - pharmacology</subject><subject>Cell Differentiation - drug effects</subject><subject>Cell Differentiation - physiology</subject><subject>Cell Survival - drug effects</subject><subject>Cell Survival - physiology</subject><subject>Cells, Cultured</subject><subject>Data Interpretation, Statistical</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Osteoblasts</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - drug effects</subject><subject>Osteoblasts - metabolism</subject><subject>Rats</subject><subject>Sheep</subject><subject>Skeleton and joints</subject><subject>Tumor Cells, Cultured - drug effects</subject><subject>Tumor Cells, Cultured - metabolism</subject><subject>Vertebrates: osteoarticular system, musculoskeletal system</subject><issn>0753-3322</issn><issn>1950-6007</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkVFrHCEQgCW0JJdLf0LKvjS0D9uOuuqal1CONC0E8pD0WTydbS17u1d1D-7f180dzWNgYND5HMdPQi4pfKZA5ZdHUILXnDP2EdgnAM50zU_IgmoBtQRQb8jiP3JGzlP6AwBC8vaUnFEmGwa8XZB-NW62NtocdliFIWNMW3QBU1nsMOXwq5TGoZojZRzXvU25clOfp4jpuvI227nosO-rXbDr0Ie8r-zgq7Qf8m_MwVXWlfZl-4K87Wyf8N0xL8nPb7dPq-_1_cPdj9XX-9o1Dc-15Yopidx3mgrZamDrhkpHlRZKUEF9K0BzKz3qDrBkJhE6aVvOuNMe-JJcHfpu4_h3Kq8wm5DmCe2A45SM4pSqVqgCigPo4phSxM5sY9jYuDcUzKzZPGs2s0MDJWbNZbUk748XTOsN-pdTR68F-HAEbHK276IdXEgvXFN-SAtRuJsDh0XHLmA0qcgfHPoQ0WXjx_DKKP8AlSWa0Q</recordid><startdate>2003</startdate><enddate>2003</enddate><creator>Torricelli, P.</creator><creator>Fini, M.</creator><creator>Giavaresi, G.</creator><creator>Borsari, V.</creator><creator>Carpi, A.</creator><creator>Nicolini, A.</creator><creator>Giardino, R.</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>2003</creationdate><title>Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity</title><author>Torricelli, P. ; Fini, M. ; Giavaresi, G. ; Borsari, V. ; Carpi, A. ; Nicolini, A. ; Giardino, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-a37276e3df91568902b416c179575151d85093a6de9f0ea6d26e0f6a8323c9d03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Calcitriol - pharmacology</topic><topic>Cell Differentiation - drug effects</topic><topic>Cell Differentiation - physiology</topic><topic>Cell Survival - drug effects</topic><topic>Cell Survival - physiology</topic><topic>Cells, Cultured</topic><topic>Data Interpretation, Statistical</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Osteoblasts</topic><topic>Osteoblasts - cytology</topic><topic>Osteoblasts - drug effects</topic><topic>Osteoblasts - metabolism</topic><topic>Rats</topic><topic>Sheep</topic><topic>Skeleton and joints</topic><topic>Tumor Cells, Cultured - drug effects</topic><topic>Tumor Cells, Cultured - metabolism</topic><topic>Vertebrates: osteoarticular system, musculoskeletal system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Torricelli, P.</creatorcontrib><creatorcontrib>Fini, M.</creatorcontrib><creatorcontrib>Giavaresi, G.</creatorcontrib><creatorcontrib>Borsari, V.</creatorcontrib><creatorcontrib>Carpi, A.</creatorcontrib><creatorcontrib>Nicolini, A.</creatorcontrib><creatorcontrib>Giardino, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biomedicine & pharmacotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Torricelli, P.</au><au>Fini, M.</au><au>Giavaresi, G.</au><au>Borsari, V.</au><au>Carpi, A.</au><au>Nicolini, A.</au><au>Giardino, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity</atitle><jtitle>Biomedicine & pharmacotherapy</jtitle><addtitle>Biomed Pharmacother</addtitle><date>2003</date><risdate>2003</risdate><volume>57</volume><issue>1</issue><spage>57</spage><epage>62</epage><pages>57-62</pages><issn>0753-3322</issn><eissn>1950-6007</eissn><coden>BIPHEX</coden><abstract>The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH)
2D
3. Cell viability was quite similar for primary osteoblast cultures (MTT: 1.64–2.11 OD); a significant (
P < 0.005) difference was found between sheep osteoblasts and MG-63 (ΔMTT: 0.52 ± 0.20 OD). Osteocalcin synthesis ranged from 15.18 to 27.00 pg/ml in primary osteoblast cultures, while it was significantly (
P < 0.01) lower in MG-63 (OC: 6.67 ± 0.52 pg/ml) when compared with primary human osteoblasts. Alkaline phosphatase, C-terminal procollagen type I, and interleukin-6 were significantly (
P < 0.005) lower in rat osteoblasts when compared with primary human osteoblasts, and similarly transforming growth factor-β1 was significantly (
P < 0.05) lower in rat and sheep osteoblasts when compared with primary human osteoblasts and MG-63. Nitric oxide synthesis did not show any significant difference either before or after vitamin 1,25(OH)
2D
3 stimulation. In conclusion, the current findings confirm the presence of interspecies differences between the selected osteoblast lineages before and after stimulation with vitamin1,25(OH)
2D
3. Above all, the culture of sheep osteoblasts was seen to behave more similarly to that of primary human cells, mainly in terms of cell viability, osteocalcin, interleukin-6 and transforming growth factor-β1 production.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>12642038</pmid><doi>10.1016/S0753-3322(02)00329-3</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0753-3322 |
| ispartof | Biomedicine & pharmacotherapy, 2003, Vol.57 (1), p.57-62 |
| issn | 0753-3322 1950-6007 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_73117857 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Calcitriol - pharmacology Cell Differentiation - drug effects Cell Differentiation - physiology Cell Survival - drug effects Cell Survival - physiology Cells, Cultured Data Interpretation, Statistical Fundamental and applied biological sciences. Psychology Humans Osteoblasts Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - metabolism Rats Sheep Skeleton and joints Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism Vertebrates: osteoarticular system, musculoskeletal system |
| title | Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T03%3A46%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20interspecies%20investigation%20on%20osteoblast%20cultures:%20data%20on%20cell%20viability%20and%20synthetic%20activity&rft.jtitle=Biomedicine%20&%20pharmacotherapy&rft.au=Torricelli,%20P.&rft.date=2003&rft.volume=57&rft.issue=1&rft.spage=57&rft.epage=62&rft.pages=57-62&rft.issn=0753-3322&rft.eissn=1950-6007&rft.coden=BIPHEX&rft_id=info:doi/10.1016/S0753-3322(02)00329-3&rft_dat=%3Cproquest_cross%3E73117857%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=73117857&rft_id=info:pmid/12642038&rft_els_id=S0753332202003293&rfr_iscdi=true |