Comparative interspecies investigation on osteoblast cultures: data on cell viability and synthetic activity

The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH) 2D 3. Cell v...

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Veröffentlicht in:	Biomedicine & pharmacotherapy 2003, Vol.57 (1), p.57-62
Hauptverfasser:	Torricelli, P., Fini, M., Giavaresi, G., Borsari, V., Carpi, A., Nicolini, A., Giardino, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Calcitriol - pharmacology Cell Differentiation - drug effects Cell Differentiation - physiology Cell Survival - drug effects Cell Survival - physiology Cells, Cultured Data Interpretation, Statistical Fundamental and applied biological sciences. Psychology Humans Osteoblasts Osteoblasts - cytology Osteoblasts - drug effects Osteoblasts - metabolism Rats Sheep Skeleton and joints Tumor Cells, Cultured - drug effects Tumor Cells, Cultured - metabolism Vertebrates: osteoarticular system, musculoskeletal system
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Zusammenfassung:	The aim of the present study was to evaluate and compare the most common parameters that characterize the expression of primary osteoblast cultures from different origin (human, rat, sheep), and of the human osteosarcoma cell line MG-63 before and after stimulation with vitamin 1,25(OH) 2D 3. Cell viability was quite similar for primary osteoblast cultures (MTT: 1.64–2.11 OD); a significant ( P
ISSN:	0753-3322 1950-6007
DOI:	10.1016/S0753-3322(02)00329-3