Production and secretion of plasminogen in cultured rat brain microglia
The production and secretion of plasminogen in cultured rat brain microglia was investigated. Urokinase-dependent cascinolytic activity was detected by zymography in microglial conditioned medium with a molecular weight of about 90 kDa. The 90-kDa protein was also detected by Western blotting with a...
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| Veröffentlicht in: | FEBS letters 1992-08, Vol.308 (2), p.179-182 |
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| creator | Nakajima, Kazuyuki Tsuzaki, Naoko Nagata, Koichi Takemoto, Nagisa Kohsaka, Shinichi |
| description | The production and secretion of plasminogen in cultured rat brain microglia was investigated. Urokinase-dependent cascinolytic activity was detected by zymography in microglial conditioned medium with a molecular weight of about 90 kDa. The 90-kDa protein was also detected by Western blotting with anti-rat plasminogen antiserum in the non-reducing condition. Immunoprecipitation with plasminogen antiserum following [
35S]methionine labelling revealed that the plasminogen detected in microglial conditioned medium is synthesized in microglia. The amount of plasminogen in the conditioned medium was increased by stimulation with lipopolysaccharide. These results show that cultured microglia produce plasminogen and secrete it into the culture medium. |
| doi_str_mv | 10.1016/0014-5793(92)81270-V |
| format | Article |
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35S]methionine labelling revealed that the plasminogen detected in microglial conditioned medium is synthesized in microglia. The amount of plasminogen in the conditioned medium was increased by stimulation with lipopolysaccharide. These results show that cultured microglia produce plasminogen and secrete it into the culture medium.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>brain</subject><subject>Brain - metabolism</subject><subject>cell culture</subject><subject>Cells, Cultured</subject><subject>Culture</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. 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Urokinase-dependent cascinolytic activity was detected by zymography in microglial conditioned medium with a molecular weight of about 90 kDa. The 90-kDa protein was also detected by Western blotting with anti-rat plasminogen antiserum in the non-reducing condition. Immunoprecipitation with plasminogen antiserum following [
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Access via ScienceDirect (Elsevier); Alma/SFX Local Collection |
| subjects | Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Blotting, Western brain Brain - metabolism cell culture Cells, Cultured Culture Electrophoresis, Polyacrylamide Gel Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Hydrolases Microglia Neuroglia - metabolism Plasminogen Plasminogen - biosynthesis Plasminogen - metabolism Plasminogen activator Precipitin Tests production Protease Rats secretion |
| title | Production and secretion of plasminogen in cultured rat brain microglia |
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