Production and secretion of plasminogen in cultured rat brain microglia

Production and secretion of plasminogen in cultured rat brain microglia

The production and secretion of plasminogen in cultured rat brain microglia was investigated. Urokinase-dependent cascinolytic activity was detected by zymography in microglial conditioned medium with a molecular weight of about 90 kDa. The 90-kDa protein was also detected by Western blotting with a...

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Veröffentlicht in:FEBS letters 1992-08, Vol.308 (2), p.179-182
Hauptverfasser: Nakajima, Kazuyuki, Tsuzaki, Naoko, Nagata, Koichi, Takemoto, Nagisa, Kohsaka, Shinichi
Format: Artikel
Sprache:eng
Schlagworte:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Blotting, Western
brain
Brain - metabolism
cell culture
Cells, Cultured
Culture
Electrophoresis, Polyacrylamide Gel
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Hydrolases
Microglia
Neuroglia - metabolism
Plasminogen
Plasminogen - biosynthesis
Plasminogen - metabolism
Plasminogen activator
Precipitin Tests
production
Protease
Rats
secretion
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Zusammenfassung:The production and secretion of plasminogen in cultured rat brain microglia was investigated. Urokinase-dependent cascinolytic activity was detected by zymography in microglial conditioned medium with a molecular weight of about 90 kDa. The 90-kDa protein was also detected by Western blotting with anti-rat plasminogen antiserum in the non-reducing condition. Immunoprecipitation with plasminogen antiserum following [ 35S]methionine labelling revealed that the plasminogen detected in microglial conditioned medium is synthesized in microglia. The amount of plasminogen in the conditioned medium was increased by stimulation with lipopolysaccharide. These results show that cultured microglia produce plasminogen and secrete it into the culture medium.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(92)81270-V