YAC mapping by FISH using Alu-PCR-generated probes

Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescen...

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Veröffentlicht in:	Genomics (San Diego, Calif.) Calif.), 1992-07, Vol.13 (3), p.726-730
Hauptverfasser:	Breen, Matthew, Arveiler, Benoit, Murray, Isobel, Gosden, John R., Porteous, David J.
Format:	Artikel
Sprache:	eng
Schlagworte:	Base Sequence Biological and medical sciences Chromosome Mapping - methods Chromosomes, Fungal Chromosomes, Human, Pair 11 Classical genetics, quantitative genetics, hybrids DNA - genetics DNA Probes Fluorescence fluorescent in situ hybridization Fundamental and applied biological sciences. Psychology Gene Library genes Genetics of eukaryotes. Biological and molecular evolution Genome, Human Human Humans mapping Molecular Sequence Data Nucleic Acid Hybridization pepsinogen A Polymerase Chain Reaction probes yeast artificial chromosomes
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Beschreibung
Zusammenfassung:	Human genomic mapping has been greatly advanced by the independent development of three new methods: large DNA fragment cloning in yeast artificial chromosomes, amplification from complex DNAs of human specific segments by Alu-PCR, and high-resolution localization of complex DNA probes by fluorescent in situ hybridization. We describe here the combination of these three analytical tools for efficient and accurate localization of randomly screened or especially selected human YAC recombinants to chromosome 11. We map a YAC clone encompassing the pepsinogen A (PGA) locus to 11q13.1–11q13.3.
ISSN:	0888-7543 1089-8646
DOI:	10.1016/0888-7543(92)90148-L