Phosphorylation of transcription factor p62TCF by MAP kinase stimulates ternary complex formation at c-fos promoter
TRANSCRIPTION of the proto-oncogene c-fos is stimulated rapidly and transiently by serum growth factors and mitogens 1 . Critical for this response is the serum-response element which is bound in vivo in a ternary complex containing the transcription factors p67 SRF and p62 TCF (ref. 2). Disruption...
Gespeichert in:
Veröffentlicht in: | Nature (London) 1992-07, Vol.358 (6385), p.414-417 |
---|---|
Hauptverfasser: | , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | TRANSCRIPTION of the proto-oncogene
c-fos
is stimulated rapidly and transiently by serum growth factors and mitogens
1
. Critical for this response is the serum-response element which is bound
in vivo
in a ternary complex containing the transcription factors p67
SRF
and p62
TCF
(ref. 2). Disruption of the ternary complex correlates with impaired induction by serum and phorbol ester
3,4
. Mitogen-activated protein (MAP) kinase is a serine/ threonine kinase which is activated 1-5 minutes after treatment of cells with mitogens and growth factors
5–8
that induce re-entry into the cell cycle, making MAP kinase a candidate for the transmission of proliferative signals. Here we show that p62
TCF
is phosphorylated by MAP kinase
in vitro
and that phosphorylation results in enhanced ternary complex formation. Serum-starved Swiss 3T3 cells treated with epidermal growth factor, which induces MAP kinase in these cells
9
, are induced to express c-
fos
and yield p62
TCF
active in ternary complex formation. In contrast, treatment of Swiss 3T3 cells with insulin, which does not activate MAP kinase under these conditions
9
, does not lead to enhanced ternary complex formation nor does it induce c-
fos
transcription. Our results link the expression of the human
c-fos
proto-oncogene to signal transduction pathways known to be activated before its own induction. |
---|---|
ISSN: | 0028-0836 1476-4687 |
DOI: | 10.1038/358414a0 |