A time-lapse capillary assay to study aerotaxis in the archaeon Halobacterium salinarum

A time-lapse capillary assay to study aerotaxis in the archaeon Halobacterium salinarum

We have developed a method for time-lapse video photography and line scanning of optical densities for analysis of aerotactic responses of Halobacterium salinarum. This automated digital technique, along with line scan analysis of selected frames, gives a unique profile of the aerotactic migration o...

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Veröffentlicht in:Journal of microbiological methods 2003-04, Vol.53 (1), p.123-126
Hauptverfasser: Boudko, Dmitri, Yu, Hyung Suk, Ruiz, Mandelle, Hou, Shaobin, Alam, Maqsudul
Format: Artikel
Sprache:eng
Schlagworte:
Air
Bacteriology
Biological and medical sciences
Capillary Action
Cells
Fundamental and applied biological sciences. Psychology
Halobacterium salinarum
Halobacterium salinarum - physiology
Image Processing, Computer-Assisted - methods
Microbiology
Microscopy, Video
Miscellaneous
Motion
Optical density
Time Factors
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Beschreibung
Zusammenfassung:We have developed a method for time-lapse video photography and line scanning of optical densities for analysis of aerotactic responses of Halobacterium salinarum. This automated digital technique, along with line scan analysis of selected frames, gives a unique profile of the aerotactic migration of halobacterial cells.
ISSN:0167-7012
1872-8359
DOI:10.1016/S0167-7012(02)00227-0