Inhibition of mouse lymphocyte proliferative response by glycosphingolipids from Leishmania (L.) amazonensis

The effect of a purified preparation of glycosphingolipids (GSLs) extracted from Leishmania (L.) amazonensis amastigotes on murine lymphocytes was investigated. GSLs inhibited both concanavalin A (Con A)- or lipopolisaccharide-induced [ 3H]thymidine uptake by normal and immunized BALB/c mouse lymph...

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Veröffentlicht in:Experimental parasitology 1992-08, Vol.75 (1), p.119-125
Hauptverfasser: Giorgio, Selma, Jasiulionis, Miriam G., Straus, Anita H., Takahashi, Helio K., Barbiéri, Clara L.
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Sprache:eng
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Zusammenfassung:The effect of a purified preparation of glycosphingolipids (GSLs) extracted from Leishmania (L.) amazonensis amastigotes on murine lymphocytes was investigated. GSLs inhibited both concanavalin A (Con A)- or lipopolisaccharide-induced [ 3H]thymidine uptake by normal and immunized BALB/c mouse lymph node cells. The effect of total GSLs was dose dependent. Total GSLs also suppressed the two-way mixed lymphocyte reaction of BALB/c × C57BL/6 cells and the antigen-specific response of immunized mouse cells. Six pure bands as well as a pool containing a mixture of GSLs with five to seven sugar residues separated by a combination of HPLC and preparative HPTLC were tested and shown to be inhibitors of Con A stimulation. These results suggest that parasite glycosphingolipids may play an immunologically relevant role in leishmaniasis.
ISSN:0014-4894
1090-2449
DOI:10.1016/0014-4894(92)90127-V